Cristin-prosjekt-ID: 2510278
Sist endret: 7. mai 2021, 15:28

Cristin-prosjekt-ID: 2510278
Sist endret: 7. mai 2021, 15:28
Prosjekt

The role of integrative conjugative elements in the spread of antibiotic resistance in Haemophilus influenzae

prosjektleder

Helene Johannessen
ved Klinikk Med Diagnostikk ved Sykehuset i Vestfold HF

prosjekteier / koordinerende forskningsansvarlig enhet

  • Sykehuset i Vestfold HF

Klassifisering

Vitenskapsdisipliner

Bioinformatikk • Medisinsk molekylærbiologi • Medisinsk mikrobiologi • Generell mikrobiologi

Emneord

Antibiotikaresistens • Biofilm • Medisinsk mikrobiologi • Mikrobiologi • Antibiotika • Antibiotika resistens

HRCS-helsekategori

  • Lunger og luftveier
  • Infeksjon
  • Generell helserelevans

HRCS-forskningsaktivitet

  • 2.1 Biologiske og indre faktorer

Kategorier

Prosjektkategori

  • Grunnforskning

Kontaktinformasjon

Telefon
97960181
Sted
Helene Johannessen

Tidsramme

Avsluttet
Start: 1. august 2021 Slutt: 31. juli 2022

Beskrivelse Beskrivelse

Tittel

The role of integrative conjugative elements in the spread of antibiotic resistance in Haemophilus influenzae

Vitenskapelig sammendrag

Resistance to antibiotics is an emerging threat to global health care, and immediate action is necessary to preserve effective antibiotics for the future. Increasing our knowledge about drivers and mechanisms of evolution and spread of antibiotic resistance is one of the four major objectives of the Norwegian national strategy to fight antibiotic resistance. Dissemination of H. influenzae with multidrug-resistance is increasing, and represents a threat to current empiric therapy.

An important mechanism for spreading of antibiotic resistance is horizontal gene transfer mediated by integrative and conjugative elements (ICEs), however the transmission and dynamics of ICEs are poorly characterized. Studies have indicated that presence of biofilm and antibiotics increases transfer of ICEs, but this has not been investigated in H. influenzae. Biofilms are multicellular microbial communities encased in a matrix and it is in these communities bacteria most often reside in clinical infections.

In order to determine the role of ICEs in the spread of antibiotic resistance in H. influenzae, we will perform mating experiments using two multidrug-resistant (MDR) clinical strains of H. influenzae with resistance genes located on ICEs. We will explore the dynamics of ICE development and evolution, determine the role of biofilm and antibiotics on the exchange of resistance genes located on ICEs between bacteria, and investigate the impact of ICEs and resistance genes on bacterial fitness.

prosjektdeltakere

prosjektleder
Aktiv cristin-person

Helene Johannessen

  • Tilknyttet:
    Prosjektleder
    ved Klinikk Med Diagnostikk ved Sykehuset i Vestfold HF

Dagfinn Skaare

  • Tilknyttet:
    Prosjektdeltaker
    ved Klinikk Med Diagnostikk ved Sykehuset i Vestfold HF
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