Cristin-prosjekt-ID: 302224
Sist endret: 28. november 2018 23:52
Cristin-prosjekt-ID: 302224
Sist endret: 28. november 2018 23:52
Prosjekt

Dissection of Epigenetic Mechanisms and Transcriptional Networks in Seed Development

prosjektleder

Paul Eivind Grini
ved Institutt for biovitenskap (tidl. IMBV) ved Universitetet i Oslo

prosjekteier / koordinerende forskningsansvarlig enhet

  • Institutt for biovitenskap (tidl. IMBV) ved Universitetet i Oslo

Finansiering

  • Norges forskningsråd

Tidsramme

Avsluttet
Start: 1. oktober 2007 Slutt: 31. desember 2011

Beskrivelse Beskrivelse

Tittel

Dissection of Epigenetic Mechanisms and Transcriptional Networks in Seed Development

Vitenskapelig sammendrag

Seed development is the product of double fertilization of the egg cell and the central cell by two sperm cells from the pollen and requires a coordinated interplay of the transcriptional programmes in the embryo, the endosperm and the maternal seed coat. Parent of origin dependent expression plays an important role in this process, and disruption of imprinted genes lead to a parental specific abortion of the seed. We will use a mutant in the Arabidopsis Cdc2/CDC28 homologue, CDKA;1 as a tool to dissect t he involvement of parental gene programs in seed development. Plants fertilized with cdka;1 mutant pollen develop viable seeds with an uniparental endosperm, given the mother is mutant for any of the FIS genes that encode Polycomb group (PcG) chromatin re modelling factors. This suggests that the action of the PcG FIS-complex balances the contribution of the paternal genome. In order to identify further factors involved in cross talk between the components of the seed and parent epigenetic regulation of se ed development, we will generate microarray transcriptional profiles of seeds with uniparental endosperm. Our preliminary data identifies several regulated genes that could represent novel paternal specific transcripts (down regulated) or maternal targets of paternal repressors (unregulated). Knock-out mutants of four members of the PcG FIS-complex will be used in combination with the cdka;1 mutant to generate transcriptional profiles and compared to wild-type and single PcG mutants. Regulated genes will be verified by real-time PCR, and knock-outs of these will generate material for new transcriptional profiles. Using the cdka;1 mutant as a tool it will for the first time it will be possible to generate transcriptional profiles from diploid, solely mater nal endosperm that supports normal embryo development. We expect that this will give an unique insight to the complexity and magnitude of imprinting and cross talk in the seed.

prosjektdeltakere

prosjektleder

Paul Eivind Grini

  • Tilknyttet:
    Prosjektleder
    ved Institutt for biovitenskap (tidl. IMBV) ved Universitetet i Oslo
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Resultater Resultater

The Genetic Basis of Post-Zygotic Hybridization Barriers Between Arabidopsis lyrata and A. arenosa.

Johannessen, Ida Marie; Hornslien, Karina Stensland; Krabberød, Anders Kristian; Bramsiepe, Jonathan; Brysting, Anne Krag; Grini, Paul Eivind. 2018, Norwegian Plant Biology 2018. UIOPoster

Pollen differentiation as well as pollen tube guidance and discharge are independent of the presence of gametes.

Glöckle, Barbara; Urban, W.; Nagahara, Shiori; Andersen, Ellen Dehnes; Higashiyama, Tetsuya; Grini, Paul Eivind; Schnittger, Arp. 2018, Development. UH, ND, UNISTRA, UIOVitenskapelig artikkel

The molecular basis of postzygotic hybridization barriers in plants.

Brysting, Anne Krag; Grini, Paul Eivind. 2017, Hybridization symposium. Hybridization: Causes and consequences for speciation. UIOVitenskapelig foredrag

The genetic basis of post-zygotic hybridization barriers between Arabidopsis lyrata and A. arenosa.

Johannessen, Ida Marie; Hornslien, Karina Stensland; Krabberød, Anders Kristian; Bramsiepe, Jonathan; Brysting, Anne Krag; Grini, Paul Eivind. 2017, The 31st Annual Meeting of the Scandinavian Pollination Ecologists (SCAPE 2017). UIOPoster

The effect of gene and genome dosage on hybridization barriers in Arabidopsis lyrata and Arabidospsis arenosa.

Johannessen, Ida Marie; Lafon-Placette, Clément; Hornslien, Karina Stensland; Bramsiepe, Jonathan; Bjerkan, Katrine; Brysting, Anne Krag; Köhler, C.; Grini, Paul Eivind. 2017, Plant Genome Evolution. UIOPoster
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