Cristin-prosjekt-ID: 303015
Sist endret: 10. januar 2017 14:01

Cristin-prosjekt-ID: 303015
Sist endret: 10. januar 2017 14:01
Prosjekt

Diversity and dynamics of marine haptophytes (HAPTODIV)

prosjektleder

Bente Edvardsen
ved Seksjon for akvatisk biologi og toksikologi ved Universitetet i Oslo

prosjekteier / koordinerende forskningsansvarlig enhet

  • Seksjon for akvatisk biologi og toksikologi ved Universitetet i Oslo

Finansiering

  • TotalbudsjettNOK 6.062.000
  • Norges forskningsråd
    Prosjektkode: 190307

Klassifisering

Emneord

Algebiologi • Marinbiologi • Marin økologi

Tidsramme

Avsluttet
Start: 1. april 2009 Slutt: 31. desember 2014

Beskrivelse Beskrivelse

Tittel

Diversity and dynamics of marine haptophytes (HAPTODIV)

Sammendrag

The primary objective is to explore the biodiversity and dynamics of haptophytes from North European coastal waters, and clarify how species composition, diversity and abundance are affected by environmental factors. From the questions addressed the following sub-goals can be put up:1. Reveal the haptophyte biodiversity in North European coastal waters. 2. Develop methodology for quantification of haptophytes from class to species level. 3. Unravel the seasonal distribution and abundance of haptophytes down to species level, and the effects of environmental factors.4. Provide new knowledge on haptophyte life cycle strategies5. Bring new strains of haptophytes and their specific viruses into culture as a resource for further studies on their taxonomy, genomics, evolution and ecology.6. Refine methods for determination of specific viruses 7. Describe distribution of haptophyte viruses in Norwegian coastal waters and correlate this to the host species composition, distribution and abundance.

Vitenskapelig sammendrag

Haptophytes are important primary producers in the marine pelagic community. Haptophyte blooms may also have a major impact on the global carbon balance and on climate forcing, and toxins may cause fish-kills. We lack knowledge on their biodiversity and quantitative distribution and dynamics at the species and population level. The main objective is to provide new knowledge on the biodiversity, seasonal dynamics of haptophytes from North Atlantic waters, and how the species composition, diversity, life cycle and abundance are affected by environmental factors. The abundance and distribution of haptophyte specific viruses will be examined, and how they affect the haptophyte diversity. In the proposed project we will take in use the latest technologies in molecular biology and microbiology and apply these to allow quantification at the species and population level of haptophytes and their viruses. The biodiversity will be explored by a.o. environmental pyrosequencing, electron microscopy and culturing. Seasonal dynamics will be examined by combining the use of microscopy, molecular probes, flow cytometry and water chemistry upon the analyses of natural samples collected from coastal waters through the year. The project will improve the understanding of the dynamics in the marine pico- and nanophytoplankton community, where haptophytes play a significant role. This will increase our understanding on fundamental processes in the plankton.
 

Metode

Cruises in coastal waters with sampling and measurements
Establish algal and viral cultures
Algal characterisation by electron microscopy and DNA-sequencing
virus characterisation by PFGE and DNA-sequencing
reveal biodiversity of haptophytes and co-occuring viruses by DNA clone libraries and 454 pyrosequencing
statistical analyses and modelling og biodiversity

Utstyr

R/V Trygve Braarud
454-Roche FLX sequencer

prosjektdeltakere

prosjektleder
Aktiv cristin-person

Bente Edvardsen

  • Tilknyttet:
    Prosjektleder
    ved Seksjon for akvatisk biologi og toksikologi ved Universitetet i Oslo

Elianne Dunthorn Egge

  • Tilknyttet:
    Prosjektdeltaker
    ved Seksjon for akvatisk biologi og toksikologi ved Universitetet i Oslo

Wenche Eikrem

  • Tilknyttet:
    Prosjektdeltaker
    ved Norsk institutt for vannforskning
  • Tilknyttet:
    Prosjektdeltaker
    ved Marinbiologi ved Universitetet i Oslo

Tom Andersen

  • Tilknyttet:
    Prosjektdeltaker
    ved Seksjon for akvatisk biologi og toksikologi ved Universitetet i Oslo

Sandra Gran Stadniczeñko

  • Tilknyttet:
    Prosjektdeltaker
    ved Seksjon for akvatisk biologi og toksikologi ved Universitetet i Oslo
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Resultater Resultater

Diversity and dynamics of marine haptophytes and their viruses (HAPTODIV).

Edvardsen, Bente. 2012, Observatoir Oceanologique de Banyuls seminar series. UIOVitenskapelig foredrag

A strategy for 454-pyrosequencing of ribosomal SSU DNA and RNA to more accurately assess protist diversity and relative abundance.

Edvardsen, Bente; Egge, Elianne Sirnæs; Bittner, Lucie; Audic, Stéphane; de Vargas, Colomban. 2012, ISOP and ISEP International conference, Protist2012. UIOVitenskapelig foredrag

Diversity of Haptophyta in the Bay of Naples as revealed by next generation sequencing (NGS): "anything new under the sea"?

Bittner, Lucie; Gobet, Angelique; Egge, Elianne Sirnæs; Audic, Stéphane; Santini, Sebastien; Ogata, Hiroyuki; Romac, Sarah; Edvardsen, Bente; Probert, Ian; de Vargas, Colomban. 2011, Jacques Monod Conference- Integrative Genomic Ecology. UIOPoster

QUANTIFICATION OF HAPTOPHYTE SPECIES BY PYROSEQUENCING: IS READ ABUNDANCE PROPORTIONAL TO SPECIES BIOVOLUME?

Edvardsen, Bente; Egge, Elianne Sirnæs. 2011, Jacques Monod Conference- Integrative Genomic Ecology. UIOPoster

Diversity and distribution of nano- and picoeukaryotes in the Southern Ocean as revealed by 454-pyrosequencing, clone libraries and microscopy.

Edvardsen, Bente; Nersveen, Lars; Torgrimsby, Tonie Leonora; Engesmo, Anette; Egge, Elianne Sirnæs; Eikrem, Wenche. 2011, International Conference, Phycological Society of America. UIOVitenskapelig foredrag
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