Cristin-prosjekt-ID: 323870
Sist endret: 14. februar 2011 18:20
Cristin-prosjekt-ID: 323870
Sist endret: 14. februar 2011 18:20
Prosjekt

Epigenetic regulation of development and growth in Senegalese sole (Solea senegalensis)- EPISOLE

prosjektleder

Luisa Valente
ved CIMAR - Centro de Investigacao Marinha e Ambiental

prosjekteier / koordinerende forskningsansvarlig enhet

  • CIMAR - Centro de Investigacao Marinha e Ambiental

Finansiering

  • Andre

Klassifisering

Vitenskapsdisipliner

Akvakultur

Emneord

Epigenetikk • Vekstregulering

Tidsramme

Avsluttet
Start: 1. februar 2011 Slutt: 1. februar 2014

Beskrivelse Beskrivelse

Tittel

Epigenetic regulation of development and growth in Senegalese sole (Solea senegalensis)- EPISOLE

Vitenskapelig sammendrag

EPISOLE will exploit genomic resources and establish a multidisciplinary group that will carry out cutting-edge research and provide state-of-the-art knowledge on the biological basis of development and growth in early life stages of fish using Senegalese sole (Solea senegalensis) as model. This innovative, timely and multidisciplinary project will involve the integration of genomic, morphological and physiological methods with nutritional and environmental effects in order to achieve improved homogeneous development, growth, and juvenile quality. During the last decade Senegalese sole has been considered one of the most promising fish species for marine aquaculture in Southern Europe. But like in other species, the sub-optimal larval rearing conditions is a bottleneck to intensive production (IV Sole workshop, Faro 2008), resulting in size dispersion, inconsistent fry quality, suboptimal growth and disease susceptibility. These problems are at least partly related to inadequate environmental and nutritional factors that affect nutrient utilization and induce poorly developed digestive and muscular systems of first feeding fish larvae. Despite the abundant information on larval morphological development, there is a lack of reliable methods and parameters to characterize and measure fundamental physiological changes. Recently developed molecular tools brought up new information on the functional ontogeny of growth, but the effect of the key environmental and nutritional factors on growth and its relation to fry quality and size variation has not yet been studied. EPISOLE will target these objectives through 5 tasks:1: Development of a molecular toolbox to study genes involved in early development and growth. The orthologues of key genes identified in other species as critical during digestive system and muscular growth differentiation will be cloned in sole to serve as potential molecular markers able to predict juvenile quality. This molecular toolbox will be applied in subsequent epigenetic studies.2: Characterization of early development and growth. The temporal and spatial expression patterns of key genes involved in digestive function and muscle growth in embryos and larvae will be described. 3: Effect of water temperature on development and growth. The impact of incubation temperature on early life history of fish will be evaluated, and its effect on protein accretion, somatic growth, ontogenesis, expression and regulation of selected genes will be described. 4: Effect of protein quality on development and growth. The effects of dietary protein hydrolysate content on digestive capacity and muscle cellularity will be integrated with the expression levels of muscle growth and digestive-related genes. 5: Polymorphism identification in the key genes involved in digestive capacity and muscle growth. To determine if the variability found on the gene expression pattern of key genes involved in digestive capacity and muscle growth is due to environmental or hereditary factors, the presence of polymorphism on the target genes will be assessed in broodstock. Additional knowledge will be added to the molecular toolbox on epigenetic regulation of digestive capacity and muscle growth-related genes, to develop a group of molecular markers to predict juvenile quality based on the findings of tasks 1-5. Integration of biological fundamental aspects derived from muscle and digestive development and their epigenetic gene regulation will allow a holistic understanding of environmental and nutritional effects on fish larvae (Senegalese sole in particular) growth potential.EPISOLE will attempt to identify new tools to be used in selection of the most adequate rearing condition able to sustain maximal growth and higher survival rates of fish larvae that can result in improved juvenile quality. This will create new knowledge for the scientific community and contribute to the sustainability of the aquaculture indust

Tittel

Epigenetic regulation of development and growth in Senegalese sole (Solea senegalensis)- EPISOLE

Vitenskapelig sammendrag

EPISOLE will exploit genomic resources and establish a multidisciplinary group that will carry out cutting-edge research and provide state-of-the-art knowledge on the biological basis of development and growth in early life stages of fish using Senegalese sole (Solea senegalensis) as model. This innovative, timely and multidisciplinary project will involve the integration of genomic, morphological and physiological methods with nutritional and environmental effects in order to achieve improved homogeneous development, growth, and juvenile quality. During the last decade Senegalese sole has been considered one of the most promising fish species for marine aquaculture in Southern Europe. But like in other species, the sub-optimal larval rearing conditions is a bottleneck to intensive production (IV Sole workshop, Faro 2008), resulting in size dispersion, inconsistent fry quality, suboptimal growth and disease susceptibility. These problems are at least partly related to inadequate environmental and nutritional factors that affect nutrient utilization and induce poorly developed digestive and muscular systems of first feeding fish larvae. Despite the abundant information on larval morphological development, there is a lack of reliable methods and parameters to characterize and measure fundamental physiological changes. Recently developed molecular tools brought up new information on the functional ontogeny of growth, but the effect of the key environmental and nutritional factors on growth and its relation to fry quality and size variation has not yet been studied. EPISOLE will target these objectives through 5 tasks:1: Development of a molecular toolbox to study genes involved in early development and growth. The orthologues of key genes identified in other species as critical during digestive system and muscular growth differentiation will be cloned in sole to serve as potential molecular markers able to predict juvenile quality. This molecular toolbox will be applied in subsequent epigenetic studies.2: Characterization of early development and growth. The temporal and spatial expression patterns of key genes involved in digestive function and muscle growth in embryos and larvae will be described. 3: Effect of water temperature on development and growth. The impact of incubation temperature on early life history of fish will be evaluated, and its effect on protein accretion, somatic growth, ontogenesis, expression and regulation of selected genes will be described. 4: Effect of protein quality on development and growth. The effects of dietary protein hydrolysate content on digestive capacity and muscle cellularity will be integrated with the expression levels of muscle growth and digestive-related genes. 5: Polymorphism identification in the key genes involved in digestive capacity and muscle growth. To determine if the variability found on the gene expression pattern of key genes involved in digestive capacity and muscle growth is due to environmental or hereditary factors, the presence of polymorphism on the target genes will be assessed in broodstock. Additional knowledge will be added to the molecular toolbox on epigenetic regulation of digestive capacity and muscle growth-related genes, to develop a group of molecular markers to predict juvenile quality based on the findings of tasks 1-5. Integration of biological fundamental aspects derived from muscle and digestive development and their epigenetic gene regulation will allow a holistic understanding of environmental and nutritional effects on fish larvae (Senegalese sole in particular) growth potential.EPISOLE will attempt to identify new tools to be used in selection of the most adequate rearing condition able to sustain maximal growth and higher survival rates of fish larvae that can result in improved juvenile quality. This will create new knowledge for the scientific community and contribute to the sustainability of the aquaculture indust

prosjektdeltakere

prosjektleder

Luisa Valente

  • Tilknyttet:
    Prosjektleder
    ved CIMAR - Centro de Investigacao Marinha e Ambiental
Aktiv cristin-person

Jorge Manuel de Oliveira Fernandes

  • Tilknyttet:
    Prosjektdeltaker
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet

Luis Conceicao

  • Tilknyttet:
    Prosjektdeltaker
    ved CIMAR - Centro de Investigacao Marinha e Ambiental

Sofia Engrola

  • Tilknyttet:
    Prosjektdeltaker
    ved CIMAR - Centro de Investigacao Marinha e Ambiental
1 - 4 av 4