Cristin-prosjekt-ID: 360831
Sist endret: 16. september 2013 10:59

Cristin-prosjekt-ID: 360831
Sist endret: 16. september 2013 10:59
Prosjekt

Viral haermorrhagic septicaemia virus (VHSV) in wild and farmed fish in Norway

prosjektleder

Renate Johansen
ved Patologi Oslo og Tromsø ved Veterinærinstituttet

prosjekteier / koordinerende forskningsansvarlig enhet

  • Patologi Oslo og Tromsø ved Veterinærinstituttet

Tidsramme

Avsluttet
Start: 1. januar 2008 Slutt: 31. desember 2013

Beskrivelse Beskrivelse

Tittel

Viral haermorrhagic septicaemia virus (VHSV) in wild and farmed fish in Norway

Sammendrag

while sea trout, wrasse etc. are couth by fishing or traps. Most of the samples are tested in cellculture, and some are tested by real time rt-PCR.VHS-virus have been detected in several herring both in Finnmark and on the west coast. This isthe first detection of VHS-virus that far north and the publication of the results on www.forskning.noresulted in some interest in the media (publication list attached).Testing of herring during the spawning season of 2010 showed a high prevalence of VHS-virusespecially in the gills. These results were presented at the EAFP-conference in Split and will soonbe published on www.forskning.no.Two papers are being prepared from the screening. One paper on the large screening and aseparate paper on the herring tested during the spawning season of 2010.ScreeningThe sampling of wild fish started in 2009 and will be finish in November 2011. So far over 2000 fishfrom 36 different fish species have been sampled. Fish like cod and herring are couth by trawling,Challenge trailsWe have succeeded in challenging cod with the VHS-virus isolate from Storfjorden. The firstchallenge trail were done by i.p. injection, and resulted in high mortality rates. Histopathology andimmunohistochemistry are being performed this fall and the paper is in preparation.A cohabitant challenge on cod has just started and willThe VHS-virus isolated from herring in Norway will be tested in a bath challenge experiment onherring in Århus in November. The EU project NADIR has provided financing of the challenge trail,and analyzing of the material will be done in the VHSV-wild-farmed-project. There have been someproblems housing the herring with high mortality rates before challenge. The challenge trail hastherefore been postponed several times.VHS-virus testingMost screening programs for VHS-virus are done by sampling several organs from 5 fish andtesting the pooled organs in cell culture. We have sampled both pooled organs from 5 fish andseparate organs in RNAlater from individual fish. This has provided us with a unique opportunityto compare these two sampling routines. The results show clearly that RT-PCR on single organsprovides a much higher prevalence of detection than pooled organs on cell culture. Gills wereonly sampled on RNAlater and tested by RT-PCR. The gills show a much higher prevalence ofVHS-virus than the internal organs (heart, kidney, spleen, brain).The challenge material from the first cod trail have been used to compare the primer set publishedby Matejusova et al 2008 and Duesund et al 2010. The Duesund primers provided a much higherdetection rate of the VHS-virus isolated from Storfjorden (genotype III). Also on other genotypes ofthe virus the new PCR shows a higher detection rate and is therefore now used in the surveillanceprogram and diagnostic services.Virulence and pathogenesisDifferent genotype III strains are being compared to possibly find virulence markers. Strains fromfish with the nervous form of VHS are compared to the kidney form.The challenge material both from cod and herring are investigated both with histopathology andimmunohistochemistry. The testing of single organs with RT-PCR has also provided interestingresults on the pathogenesis.EpidemiologyA risk-based surveillance system has been established and the model has been presented onseveral international meetings. The paper is in preparation.

Vitenskapelig sammendrag

Prosjektets hovedmål og delmålThe primary objective of the project is to gain knowledge on the prevalence of VHSV in wild and farmed fish species and the risk of transmission of virus between different fish populations.Secondary objectives 1. Screening and genotyping of coastal wild fish populations for VHSV with emphasis on fish species shown to be susceptible for VHSV infection. Material will be stored for possible use in future screening also of other infectious agents. 2. Identification of possible genetic virulence markers for VHS 3. Experimental studies of the pathogenicity of different VHSV strainsfor various fish species 4. Establishment of an epidemiological database on VHSV 5. Develop a risk-based surveillance system for VHVS in wild and farmed fish populations

prosjektdeltakere

prosjektleder
Inaktiv cristin-person

Renate Johansen

  • Tilknyttet:
    Prosjektleder
    ved Patologi Oslo og Tromsø ved Veterinærinstituttet

Nina Sandlund

  • Tilknyttet:
    Prosjektdeltaker

Øivind Bergh

  • Tilknyttet:
    Prosjektdeltaker

Britt Gjerset

  • Tilknyttet:
    Prosjektdeltaker

Ingebjørg Modahl

  • Tilknyttet:
    Prosjektdeltaker
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Resultater Resultater

ATLANTIC HERRING SHOWS HIGH MORTALITY RATE IN BATH CHALLENGE WITH VIRAL HAEMORRHAGIC SEPTICAEMIA VIRUS (VHSV).

Johansen, Renate; Bergh, Øivind; Gjerset, Britt; Modahl, Ingebjørg; Sandlund, Nina. 2013, EAFP Tampere 2013. VETINST, HAVFORSKPoster

High prevalence of viral haemorrhagic septicaemia virus (VHSV) in Norwegian spring-spawning herring.

Johansen, Renate; Bergh, Øivind; Modahl, Ingebjørg; Dahle, Geir; Gjerset, Britt; Holst, Jens Christian; Sandlund, Nina. 2013, Marine Ecology Progress Series. VETINST, HAVFORSKVitenskapelig artikkel

Fant mye VHS-virus i sild.

Johansen, Renate; Gjerset, Britt. 2011, VETINSTNettsider (opplysningsmateriale)

Viral Hemoragisk Septikemi Virus (VHSV) langs Norskekysten.

Sandlund, Nina; Johansen, Renate; Bergh, Øivind. 2010, Fiskehelsebladet. VETINST, HAVFORSKPopulærvitenskapelig artikkel

Undersøkelser for Viral hemoragisk septikemi virus (VHS-virus) i villfisk i Norge.

Sandlund, Nina; Johansen, Renate; Tarpai, Attila; Bergh, Øivind. 2010, Havbrukskonferansen. VETINST, HAVFORSKPoster
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