Cristin-prosjekt-ID: 405148
Sist endret: 21. januar 2014 14:32
Cristin-prosjekt-ID: 405148
Sist endret: 21. januar 2014 14:32
Prosjekt

Nevrofysiologi ved reproduksjon

prosjektleder

Trude Marie Haug
ved Universitetet i Oslo

prosjekteier / koordinerende forskningsansvarlig enhet

  • Havforskningsinstituttet
  • Universitetet i Oslo

Klassifisering

Vitenskapsdisipliner

Cellebiologi

Emneord

Torsk

Tidsramme

Avsluttet
Start: 1. mars 2010 Slutt: 31. desember 2012

Beskrivelse Beskrivelse

Tittel

Nevrofysiologi ved reproduksjon

Sammendrag

Forskningsprosjektet er rettet mot de ulike fysiologiske mekanismene som starter og terminerer pubertet. Vi bruker fiskene Atlantic cod (Gadus morhua) og Medaka (Oryzias latipes) som forsøksorganismer.

Vitenskapelig sammendrag

Forskningsprosjektet er rettet mot de ulike fysiologiske mekanismene som starter og terminerer pubertet. Vi bruker fiskene Atlantic cod (Gadus morhua) og Medaka (Oryzias latipes) som forsøksorganismer.

Tittel

Nevrophysiology of reproduction

Sammendrag

Puberty and sexual maturation in vertebrates are regulated through increased activity in the brain-pituitary-gonad axis. Stimulatory and inhibitory inputs merge in the forebrain on neuroendocrine neurons that produce gonadotropin-releasing hormones (GnRHs). Upon stimulation, FSH and LH are released independently of each other into the circulation, and each stimulates different stages of gonadal development. The key factors in pubertal development probably include increased release of GnRH and maturation of gonadotropes, e.g. adequate expression of GnRH receptors and components of intracellular signalling pathways. However, how this is regulated and the triggering mechanism(s) for puberty are not known. The proposed experiments may answer these questions in fish, and imply similar answers for vertebrates in general. We believe that teleost fish are useful models, since they have separate cells producing FSH and LH. We have developed relevant methodologies, like primary pituitary cultures suitable for electrophysiological recordings. This project aims to use transgenic medaka lines that express GFP in gonadotropes as a new model system. The transgenic fish will enable us to identify and isolate FSH- and LH-producing gonadotropes, respectively. In parallel, we will perform similar studies on Atlantic cod, a species we already have started to characterize. In this species, we will be able to identify FSH and LH-producing cells through single-cell PCR. The identified cells will be used in electrophysiological analyses as well as Ca2+-imaging studies to investigate properties of the two different gonadotropes at various stages of sexual maturation and upon exposure to various endogenous factors. We will simultaneously monitor changes in both membrane properties and [Ca2+]i during the response to different versions of the GnRH peptide, activators of putative messengers, like PKA, PKC and cAMP, and agonists/antagonists to the GnRH-R(s).

Vitenskapelig sammendrag

Puberty and sexual maturation in vertebrates are regulated through increased activity in the brain-pituitary-gonad axis. Stimulatory and inhibitory inputs merge in the forebrain on neuroendocrine neurons that produce gonadotropin-releasing hormones (GnRHs). Upon stimulation, FSH and LH are released independently of each other into the circulation, and each stimulates different stages of gonadal development. The key factors in pubertal development probably include increased release of GnRH and maturation of gonadotropes, e.g. adequate expression of GnRH receptors and components of intracellular signalling pathways. However, how this is regulated and the triggering mechanism(s) for puberty are not known. The proposed experiments may answer these questions in fish, and imply similar answers for vertebrates in general. We believe that teleost fish are useful models, since they have separate cells producing FSH and LH. We have developed relevant methodologies, like primary pituitary cultures suitable for electrophysiological recordings. This project aims to use transgenic medaka lines that express GFP in gonadotropes as a new model system. The transgenic fish will enable us to identify and isolate FSH- and LH-producing gonadotropes, respectively. In parallel, we will perform similar studies on Atlantic cod, a species we already have started to characterize. In this species, we will be able to identify FSH and LH-producing cells through single-cell PCR. The identified cells will be used in electrophysiological analyses as well as Ca2+-imaging studies to investigate properties of the two different gonadotropes at various stages of sexual maturation and upon exposure to various endogenous factors. We will simultaneously monitor changes in both membrane properties and [Ca2+]i during the response to different versions of the GnRH peptide, activators of putative messengers, like PKA, PKC and cAMP, and agonists/antagonists to the GnRH-R(s).

prosjektdeltakere

prosjektleder

Trude Marie Haug

  • Tilknyttet:
    Prosjektleder
    ved Universitetet i Oslo

Heidi Kristine Grønlien

  • Tilknyttet:
    Prosjektdeltaker
    ved Avdeling for helse og velferd ved Høgskolen i Østfold

Finn-Arne Weltzien

  • Tilknyttet:
    Prosjektdeltaker
    ved Havforskningsinstituttet

Olav Sand

  • Tilknyttet:
    Prosjektdeltaker
    ved Universitetet i Oslo
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