Cristin-prosjekt-ID: 411992
Sist endret: 2. november 2016 10:10
Cristin-prosjekt-ID: 411992
Sist endret: 2. november 2016 10:10
Prosjekt

FishmiR - Role of small non-coding RNA in fish development: comparative study on regulatory mechanisms

prosjektleder

Igor Szczepan Babiak
ved Fakultet for biovitenskap og akvakultur ved Nord universitet

prosjekteier / koordinerende forskningsansvarlig enhet

  • Fakultet for biovitenskap og akvakultur ved Nord universitet

Finansiering

  • TotalbudsjettNOK 2.200.000
  • Norges forskningsråd

    • Prosjektkode: 213825

Kontaktinformasjon

Tidsramme

Avsluttet
Start: 15. november 2012 Slutt: 31. desember 2015

Beskrivelse Beskrivelse

Tittel

FishmiR - Role of small non-coding RNA in fish development: comparative study on regulatory mechanisms

Populærvitenskapelig sammendrag

The major purpose of this project is to have an insight into evolution, biogenesis and functions of various regulatory non-coding RNA (ncRNA) in fish development. We will analysenCRNA transcriptome in fish representing distant phygogenetic lineages, inclu ding representatives of 4 out of 5 superorders of Teleostei (zebrafish, Atlantic salmon, Atlantic cod, and Atlantic halibut), one Chondrostei (white sturgeon), and one Holostei (spotted gar, being an outgroup to both Telostei and Chondrostei in term os wh ole genome duplication events. Our approach is to generate ncRNA transcriptome databases using next generation sequencing technologies, identify conservations and variations across the evolutionary lineages, and perform functional analyses to learn abo ut regulatory functions in developmental processes. Emphasis will be given to: germline development, maternal-zygotic regulatory transition, and parental contribution

Vitenskapelig sammendrag

We will investigate the distribution, evolutionary conservation, and properties
of small, non-protein coding RNAs (ncRNAs), particularly microRNAs
(miRNAs), which are important regulatory components of developmental
processes and have biological roles and mechanisms of action that are to a
great extent as yet undiscovered. Our approach involves a combination of
computational and functional studies, including high-throughput sequencing
and cutting edge functional genomics tools. We will involve national
state-of-the art infrastructure resources (SOLiD platform and Zebrafish
Platform) and international top-level expertise in vertebrate genome
evolution.
Ray-finned (Actinopterygii) fishes representing distant phylogenetic
lineages are chosen: Teleostei, zebrafish (superorder: Ostariophysi),
Atlantic cod (superorder: Paracanthopterygii), Atlantic halibut (superorder:
Acanthopterygii), and spotted gar (Holostei), a living fossil, serving as an
outgroup that diverged from the three other species before the teleost
genome duplication event. Comparative study performed on the four species
will give reliable insight to evaluate principles that are universal or species
specific for the investigated processes.
We will focus on features that are so far poorly investigated, such as
occurrence of size variants in miRNAs and their differential patterning; the
unknown role of miRNA 3-prime-end modifications; and the occurrence of
very short ncRNA sequences in transcriptome datasets. Also, we will focus
on germline-specific ncRNAs to elucidate their role in germline specification
with an applicative outcome to control reproduction in aquaculture species.
The proposed research addresses fundamental questions in biology and
medicine related to regulatory mechanisms of biological processes. The
project will generate new knowledge on ncRNA abundance, distribution,
evolutionary conservation and germline development. The comparative study
implies potential impact on farm fish species.

prosjektdeltakere

prosjektleder

Igor Szczepan Babiak

  • Tilknyttet:
    Prosjektleder
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet

Steinar Daae Johansen

  • Tilknyttet:
    Prosjektdeltaker
    ved Nord universitet

Peter Alestrøm

  • Tilknyttet:
    Prosjektdeltaker
    ved Norges miljø- og biovitenskapelige universitet

Rebecca Roby

  • Tilknyttet:
    Prosjektdeltaker
Aktiv cristin-person

Jorge Manuel de Oliveira Fernandes

  • Tilknyttet:
    Prosjektdeltaker
    ved Nord universitet
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