Cristin-prosjekt-ID: 490817
Sist endret: 31. oktober 2017 22:55

Cristin-prosjekt-ID: 490817
Sist endret: 31. oktober 2017 22:55
Prosjekt

Development of cost efficient advanced DNA-based methods for specific traceability issues and high level on-site applications

prosjektleder

Arne Holst-Jensen
ved Forskningsgruppe dyrehelse ved Veterinærinstituttet

prosjekteier / koordinerende forskningsansvarlig enhet

  • Veterinærinstituttet

Finansiering

  • EU
    Prosjektkode: FP7-KBBE-2013-7-613908-DECATHLON

Klassifisering

Vitenskapsdisipliner

Bioinformatikk • Molekylærbiologi

Emneord

Bakteriologi • Genmodifiserte planter • Næringsmiddelanalyse • Artsmangfold • Patogenitet • truede arter • toll- og avgiftsformål

Kategorier

Prosjektkategori

  • Anvendt forskning

Tidsramme

Avsluttet
Start: 1. desember 2013 Slutt: 30. november 2016

Beskrivelse Beskrivelse

Tittel

Development of cost efficient advanced DNA-based methods for specific traceability issues and high level on-site applications

Vitenskapelig sammendrag

The Decathlon project’s strategic objective is the development of cost efficient advanced DNA-based methods for specific traceability issues and high level on-site applications. More specific DECATHLON aims to: Develop advanced, dedicated DNA-based methodologies for lab-based and on-site application for selected issues in the fields of 1) food pathogens, 2) traceability of GMOs, and 3) customs issues; Develop and apply minimal performance parameters (MPPs) for DNA extraction, isothermal amplification, different PCR-based approaches and next-generation sequencing (NGS) with emphasis on cost efficiency; Include socio-economic factors and other impact criteria in the development of methods, such as on-site applicability and equivalence to current standard approaches; Demonstrate the advanced characteristics of developed methods that are important for decision making, including measurement uncertainties under suboptimal conditions; Developtraining schemes for inter-laboratory validation trials and facilitate subsequent application of the developed methods by additional laboratories on the basis of e-learning tools.

The Decathlon project has already initiated its activities and as outcomes the following overall result is foreseen:

  • Support the development of informative and cost-effective DNA-based methods for detection, identification quantification and characterization of foodborne pathogens and GMOs. Similar methodologies will be developed for the most relevant issues defined by the European customs laboratories to address current analytical shortcomings. 
  • For each of the three listed areas more than one highly sensitive detection and identification tool will be developed that will form a better basis for decision making. The impact of this will in principle be major improvements with respects to quality, safety, identification and traceability of bioeconomy products. 

 

prosjektdeltakere

prosjektleder
Aktiv cristin-person

Arne Holst-Jensen

  • Tilknyttet:
    Prosjektleder
    ved Forskningsgruppe dyrehelse ved Veterinærinstituttet

Anja Bråthen Kristoffersen

  • Tilknyttet:
    Prosjektdeltaker
    ved Epidemiologi ved Veterinærinstituttet

Karin Lagesen

  • Tilknyttet:
    Prosjektdeltaker
    ved Epidemiologi ved Veterinærinstituttet

Camilla Sekse

  • Tilknyttet:
    Prosjektdeltaker
    ved Mattrygghet og nye helsetrusler ved Veterinærinstituttet

Gro Johannessen

  • Tilknyttet:
    Prosjektdeltaker
    ved Mattrygghet og nye helsetrusler ved Veterinærinstituttet
1 - 5 av 6 | Neste | Siste »

Resultater Resultater

High throughput sequencing for detection of foodborne pathogens.

Sekse, Camilla; Holst-Jensen, Arne; Dobrindt, Ulrich; Johannessen, Gro Skøien; Li, Weihua; Spilsberg, Bjørn; Shi, Jianxin. 2017, Frontiers in Microbiology. VETINST, WWU, SJTUVitenskapelig oversiktsartikkel/review

Development and validation of a multi-locus DNA metabarcoding method to identify endangered species in complex samples.

Arulandhu, Alfred J.; Staats, Martijn; Hagelaar, Rico; Voorhuijzen, Marleen; Prins, Theo W.; Scholtens, Ingrid; Costessi, Adalberto; Duijsing, Dany; Rechenmann, François; Gaspar, Frédéric mfl.. 2017, GigaScience. PORTUGAL, USA, STORBRITAN, AGES, TSJEKKIA, FRANKRIKE, VETINST, WUR, SVEITS, BELGIA, TYSKLAND, SLV, NEDERLAND, ITALIA, EUROFINSVitenskapelig artikkel

Development and inter-laboratory assessment of droplet digital PCR assays for multiplex quantification of 15 genetically modified soybean lines.

Kosir, Alexandra Bogozalec; Spilsberg, Bjørn; Holst-Jensen, Arne; Zel, Jana; Dobnik, David. 2017, Scientific Reports. VETINST, SLOVAKIAVitenskapelig artikkel

Identification and quantification of genetically modified organisms (GMO) from high throughput sequencing data .

Spilsberg, Bjørn; Lagesen, Karin; Kristoffersen, Anja Bråthen; Holst-Jensen, Arne. 2017, qPCR dPCR & NGS 2017. VETINST, UIOPoster

DNA extraction from food matrixes omitting pre-enrichment.

Spilsberg, Bjørn; Johannesen, Gro; Dreo, Tanja; Seyfarth, Ralf. 2016, Rapid Methods Europe 11th conference. VETINSTVitenskapelig foredrag
1 - 5 av 33 | Neste | Siste »