Cristin-resultat-ID: 1039515
Sist endret: 4. november 2013, 15:44
NVI-rapporteringsår: 2013
Resultat
Vitenskapelig artikkel
2013

LC-MS Analysis with Thiol Derivatization to Differentiate [Dhb(7)]- from [Mdha(7)]-Microcystins: Analysis of Cyanobacterial Blooms, Planktothrix Cultures and European Crayfish from Lake Steinsfjorden, Norway

Bidragsytere:
  • Christopher Owen Miles
  • Morten Sandvik
  • Sigrid Haande
  • Hezron Emmanuel Nonga og
  • Andreas Ballot

Tidsskrift

Environmental Science and Technology
ISSN 0013-936X
e-ISSN 1520-5851
NVI-nivå 2

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2013
Volum: 47
Hefte: 9
Sider: 4080 - 4087

Importkilder

Isi-ID: 000318756000018

Klassifisering

Emneord

Fisk

Beskrivelse Beskrivelse

Tittel

LC-MS Analysis with Thiol Derivatization to Differentiate [Dhb(7)]- from [Mdha(7)]-Microcystins: Analysis of Cyanobacterial Blooms, Planktothrix Cultures and European Crayfish from Lake Steinsfjorden, Norway

Sammendrag

Kinetic studies showed that [Asp3, Dhb7]MC-RR reacted with mercaptoethanol hundreds of times more slowly than MC-RR and a range of other [Mdha7]-containing microcystin congeners. The difference in reaction rate was sufficiently large that derivatization of microcystin-containing samples with mercaptoethanol, followed by LC-MS analysis, clearly discriminated between microcystins containing the isobaric [Dhb7]- and [Mdha7]-groups. Application of this approach, using LC-MS with both-ion trap and triplequadrupole mass spectrometers, to water samples and Planktothrix cultures from Lake Steinsfjorden, Norway, demonstrated the presence of [Asp3, Dhb7]MC-RR (5), [Asp3]MC-RY (14), and [Asp3]MC-LY (16), as well as analogues tentatively identified as [Asp3]MC-RR (4), [Asp3, DMAdda5, Dhb7]MC-LR (6), [Asp3, Dhb7]MC-HtyR (8), [Asp3]MC-HtyR (9), [Asp3, Dhb7]MC-LR (10), [Asp3]MC-LR (11), [Asp3, Dhb7]MC-RY (15), and [Asp3, Dhb7]MC-LY (17), together with low levels of several other analogues. This is the first use of this thiol-based LC-MS approach to identify Dhb-containing microcystins, and allowed identification of LC-MS peaks in a mixture of [Mdha7]- and [Dhb7]-congeners of [Asp3]MC-RR (4, 5), -RY (14, 15), and -LY (16, 17) in the samples from L. Steinsfjorden. This is also the first report of MC-RY-congeners outside of Africa, or in Planktothrix spp. Analysis of European crayfish (Astacus astacus) taken from L. Steinsfjorden revealed the presence of only trace levels of microcystins in the edible parts.

Bidragsytere

Christopher Owen Miles

  • Tilknyttet:
    Forfatter
    ved Kjemi ved Veterinærinstituttet

Morten Sandvik

  • Tilknyttet:
    Forfatter
    ved Kjemi ved Veterinærinstituttet

Sigrid Haande

  • Tilknyttet:
    Forfatter
    ved Ferskvannsøkologi ved Norsk institutt for vannforskning

Hezron Emmanuel Nonga

  • Tilknyttet:
    Forfatter
    ved Kjemi ved Veterinærinstituttet
  • Tilknyttet:
    Forfatter
    ved Sokoine University of Agriculture

Andreas Hendrik Ballot

Bidragsyterens navn vises på dette resultatet som Andreas Ballot
  • Tilknyttet:
    Forfatter
    ved Kjemi ved Veterinærinstituttet
  • Tilknyttet:
    Forfatter
    ved Ferskvannsøkologi ved Norsk institutt for vannforskning
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