Cristin-resultat-ID: 1062114
Sist endret: 30. oktober 2017, 11:03
NVI-rapporteringsår: 2013
Resultat
Vitenskapelig artikkel
2014

The microbial communities in two apparently physically separated deep subsurface oil reservoirs show extensive DNA sequence similarities

Bidragsytere:
  • Anna Lewin
  • Jostein Johansen
  • Alexander Wentzel
  • Hans Kristian Kotlar
  • Finn Drabløs og
  • Svein Valla

Tidsskrift

Environmental Microbiology
ISSN 1462-2912
e-ISSN 1462-2920
NVI-nivå 2

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2014
Publisert online: 2013
Trykket: 2014
Volum: 16
Hefte: 2
Sider: 545 - 558

Importkilder

Scopus-ID: 2-s2.0-84892954948

Beskrivelse Beskrivelse

Tittel

The microbial communities in two apparently physically separated deep subsurface oil reservoirs show extensive DNA sequence similarities

Sammendrag

It is well established that micro-organisms colonize a variety of extreme environments, including habitats like oil reservoirs deep inside the earth crust. Here, we present the results of a comparative high-coverage DNA sequencing study of metagenomes derived from two different oil reservoirs, both located about 2.5 km subseafloor below the Norwegian Sea. A previously reported bioinformatic analysis of DNA sequence data derived from one of the reservoirs (Well I) indicated that the community is dominated by bacterial species with a smaller fraction of Archaea. Here, we report results of a similar analysis from another reservoir (Well II) located in the same geographical area, however, according to available geological knowledge lacking direct physical contact with Well I. Interestingly, the Well II community is largely dominated by Archaea with a subordinate fraction of Bacteria. Comparison of the two datasets showed that large fractions of the sequences are extremely similar, both with respect to identity (typically above 98%) and gene organization. We therefore conclude that both wells contain essentially the same organisms, but in different relative abundances. Assuming that the communities have been distinct for long timescales because of physical separation, the results also indicate that microbial growth in the reservoirs is extremely slow.

Bidragsytere

Anna Lewin

  • Tilknyttet:
    Forfatter
    ved Institutt for bioteknologi og matvitenskap ved Norges teknisk-naturvitenskapelige universitet

Jostein Johansen

  • Tilknyttet:
    Forfatter
    ved Institutt for klinisk og molekylær medisin ved Norges teknisk-naturvitenskapelige universitet
Aktiv cristin-person

Alexander Wentzel

  • Tilknyttet:
    Forfatter
    ved Bioteknologi og nanomedisin ved SINTEF AS

Hans Kristian Kotlar

  • Tilknyttet:
    Forfatter
    ved Equinor

Finn Drabløs

  • Tilknyttet:
    Forfatter
    ved Institutt for klinisk og molekylær medisin ved Norges teknisk-naturvitenskapelige universitet
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