Cristin-resultat-ID: 1139254
Sist endret: 25. oktober 2016, 14:39
Resultat
Poster
2014

Short term changes in marine bacterial community structure following crude oil exposure at low temperature

Bidragsytere:
  • Adriana Krolicka
  • Catherine Boccadoro
  • Mari Mæland og
  • Thierry Baussant

Presentasjon

Navn på arrangementet: 15th International Symposium on Microbial Ecology
Dato fra: 24. august 2014
Dato til: 29. august 2014

Om resultatet

Poster
Publiseringsår: 2014

Beskrivelse Beskrivelse

Tittel

Short term changes in marine bacterial community structure following crude oil exposure at low temperature

Sammendrag

Temperature is one of the key factors influencing crude oil biodegradation rate. According to the central biochemical dogma, metabolic processes are thought to increase with temperature, and numerous papers have indicated a slower crude oil biodegradation rate in polar and sub-polar compared to more temperate regions. However, recent publications have described cases of even higher hydrocarbon biodegradation rates at temperatures close to the freezing point. Cold- adapted microorganisms seem to possess an intrinsic advantage in the efficient degradation of contaminants at low temperature. The goals of this study were; 1) to track short term changes in the marine bacterial community structure following crude oil exposure at low temperature (4oC) and 2) to taxonomical identify the organisms enriched in the early stages of the exposure. The experiment investigated the microbial communities in seawater for 28 days following crude oil exposures with and without added dispersant, with particular focus within the first two days. Analysis of 16S rDNA sequences created by 454 amplicon sequencing with Lib-L library indicated a significantly higher numbers for some ribotypes (Pseudomonas, Pseudoalteromonas, Marinobacter, Marinomonas, Polaribacter, Shinogomonodaceae) after 24 h exposure compared to the non-exposed reference sample. These pyrosequencing results were supported by 16S rDNA PCR DGGE, with both methods indicating substantial changes in the bacterial community composition, and the sequencing of unique DGGE gel bands corresponding to species also identified by the 454 sequencing as enriched in crude oil exposed samples. These findings indicate shifts in the marine microbial community structure and diversity within 24 hours of the introduction of crude oil into the environment, with the growth of particular hydrocarbon degrading organisms being strongly stimulated despite the low exposure temperature of 4oC

Bidragsytere

Adriana Krolicka

  • Tilknyttet:
    Forfatter
    ved NORCE Klima og miljø ved NORCE Norwegian Research Centre AS

Catherine Boccadoro

  • Tilknyttet:
    Forfatter
    ved NORCE Klima og miljø ved NORCE Norwegian Research Centre AS

Mari Mæland Nilsen

Bidragsyterens navn vises på dette resultatet som Mari Mæland
  • Tilknyttet:
    Forfatter
    ved NORCE Klima og miljø ved NORCE Norwegian Research Centre AS

Thierry Baussant

  • Tilknyttet:
    Forfatter
    ved NORCE Klima og miljø ved NORCE Norwegian Research Centre AS
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