Cristin-resultat-ID: 1177446
Sist endret: 22. januar 2015, 12:09
NVI-rapporteringsår: 2014
Resultat
Vitenskapelig artikkel
2014

Development and Validation of a Gene Profile Predicting Benefit of Postmastectomy Radiotherapy in Patients with High-Risk Breast Cancer: A Study of Gene Expression in the DBCG82bc Cohort

Bidragsytere:
  • Trine Tramm
  • Hayat Mohammed
  • Simen Myhre
  • Marianne Kyndi
  • Jan Alsner
  • Anne-Lise Børresen-Dale
  • mfl.

Tidsskrift

Clinical Cancer Research
ISSN 1078-0432
e-ISSN 1557-3265
NVI-nivå 2

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2014
Publisert online: 2014
Volum: 20
Hefte: 20
Sider: 5272 - 5280
Open Access

Importkilder

Scopus-ID: 2-s2.0-84909619045

Beskrivelse Beskrivelse

Tittel

Development and Validation of a Gene Profile Predicting Benefit of Postmastectomy Radiotherapy in Patients with High-Risk Breast Cancer: A Study of Gene Expression in the DBCG82bc Cohort

Sammendrag

Purpose: To identify genes predicting benefit of radiotherapy in patients with high-risk breast cancer treated with systemic therapy and randomized to receive or not receive postmastectomy radiotherapy (PMRT). Experimental Design: The study was based on the Danish Breast Cancer Cooperative Group (DBCG82bc) cohort. Gene-expression analysis was performed in a training set of frozen tumor tissue from 191 patients. Genes were identified through the Lasso method with the endpoint being locoregional recurrence (LRR). A weighted gene-expression index (DBCG-RT profile) was calculated and transferred to quantitative real-time PCR (qRT-PCR) in corresponding formalin-fixed, paraffin-embedded (FFPE) samples, before validation in FFPE from 112 additional patients. Results: Seven genes were identified, and the derived DBCG-RT profile divided the 191 patients into “high LRR risk” and “low LRR risk” groups. PMRT significantly reduced risk of LRR in “high LRR risk” patients, whereas “low LRR risk” patients showed no additional reduction in LRR rate. Technical transfer of the DBCG-RT profile to FFPE/qRT-PCR was successful, and the predictive impact was successfully validated in another 112 patients. Conclusions: A DBCG-RT gene profile was identified and validated, identifying patients with very low risk of LRR and no benefit from PMRT. The profile may provide a method to individualize treatment with PMRT

Bidragsytere

Trine Tramm

  • Tilknyttet:
    Forfatter
    ved Århus Universitetshospital

Hayat Mohammed

  • Tilknyttet:
    Forfatter
    ved Avdeling for biostatistikk ved Universitetet i Oslo

Simen Myhre

  • Tilknyttet:
    Forfatter
    ved K.G.Jebsen senter for brystkreftforsking ved Universitetet i Oslo
  • Tilknyttet:
    Forfatter
    ved Institutt for kreftforskning ved Universitetet i Oslo
  • Tilknyttet:
    Forfatter
    ved Seksjon for kreftgenetikk ved Oslo universitetssykehus HF
  • Tilknyttet:
    Forfatter
    ved Atlantis Medisinske Høgskole

Marianne Kyndi

  • Tilknyttet:
    Forfatter
    ved Århus Universitetshospital

Jan Alsner

  • Tilknyttet:
    Forfatter
    ved Århus Universitetshospital
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