Cristin-resultat-ID: 1196333
Sist endret: 16. mars 2015, 10:15
NVI-rapporteringsår: 2014
Resultat
Vitenskapelig artikkel
2014

Methylotrophy in the thermophilic Bacillus methanolicus, basic insights and application for commodity production from methanol

Bidragsytere:
  • Jonas E.N. Müller
  • Tonje Marita Bjerkan Heggeset
  • Volker F. Wendisch
  • Julia A. Vorholt og
  • Trygve Brautaset

Tidsskrift

Applied Microbiology and Biotechnology
ISSN 0175-7598
e-ISSN 1432-0614
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2014
Publisert online: 2014
Trykket: 2015
Volum: 99
Hefte: 2
Sider: 535 - 551

Importkilder

Scopus-ID: 2-s2.0-84912551818
Scopus-ID: 2-s2.0-84925462820

Beskrivelse Beskrivelse

Tittel

Methylotrophy in the thermophilic Bacillus methanolicus, basic insights and application for commodity production from methanol

Sammendrag

Using methanol as an alternative non-food feedstock for biotechnological production offers several advantages in line with a methanol-based bioeconomy. The Gram-positive, facultative methylotrophic and thermophilic bacterium Bacillus methanolicus is one of the few described microbial candidates with a potential for the conversion of methanol to value-added products. Its capabilities of producing and secreting the commercially important amino acids l-glutamate and l-lysine to high concentrations at 50 °C have been demonstrated and make B. methanolicus a promising target to develop cell factories for industrial-scale production processes. B. methanolicus uses the ribulose monophosphate cycle for methanol assimilation and represents the first example of plasmid-dependent methylotrophy. Recent genome sequencing of two physiologically different wild-type B. methanolicus strains, MGA3 and PB1, accompanied with transcriptome and proteome analyses has generated fundamental new insight into the metabolism of the species. In addition, multiple key enzymes representing methylotrophic and biosynthetic pathways have been biochemically characterized. All this, together with establishment of improved tools for gene expression, has opened opportunities for systems-level metabolic engineering of B. methanolicus. Here, we summarize the current status of its metabolism and biochemistry, available genetic tools, and its potential use in respect to overproduction of amino acids.

Bidragsytere

Jonas E.N. Müller

  • Tilknyttet:
    Forfatter
    ved Eidgenössische Technische Hochschule Zürich
Aktiv cristin-person

Tonje Marita Bjerkan Heggeset

  • Tilknyttet:
    Forfatter
    ved Bioteknologi og nanomedisin ved SINTEF AS

Volker F. Wendisch

  • Tilknyttet:
    Forfatter
    ved Universität Bielefeld

Julia A. Vorholt

  • Tilknyttet:
    Forfatter
    ved Eidgenössische Technische Hochschule Zürich

Trygve Brautaset

  • Tilknyttet:
    Forfatter
    ved Bioteknologi og nanomedisin ved SINTEF AS
  • Tilknyttet:
    Forfatter
    ved Institutt for bioteknologi og matvitenskap ved Norges teknisk-naturvitenskapelige universitet
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