Cristin-resultat-ID: 1244658
Sist endret: 23. februar 2016, 11:37
NVI-rapporteringsår: 2015
Resultat
Vitenskapelig artikkel
2015

Syndecan-4 regulates muscle differentiation and is internalized from the plasma membrane during myogenesis

Bidragsytere:
  • Sissel Beate Rønning
  • Cathrine Rein Carlson
  • Espen Stang
  • Svein Olav Kolset
  • Kristin Hollung og
  • Mona Elisabeth Pedersen

Tidsskrift

PLOS ONE
ISSN 1932-6203
e-ISSN 1932-6203
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2015
Publisert online: 2015
Volum: 10
Hefte: 6
Open Access

Importkilder

Scopus-ID: 2-s2.0-84935481455

Beskrivelse Beskrivelse

Tittel

Syndecan-4 regulates muscle differentiation and is internalized from the plasma membrane during myogenesis

Sammendrag

The cell surface proteoglycan syndecan-4 has been reported to be crucial for muscle differentiation, but the molecular mechanisms still remain to be fully understood. During in vitro differentiation of bovine muscle cells immunocytochemical analyses showed strong labelling of syndecan-4 intracellularly, in close proximity with Golgi structures, in membranes of intracellular vesicles and finally, in the nuclear area including the nuclear envelope. Chase experiments showed that syndecan-4 was internalized from the plasma membrane during this process. Furthermore, when syndecan-4 was knocked down by siRNA more myotubes were formed, and the expression of myogenic transcription factors, β1-integrin and actin was influenced. However, when bovine muscle cells were treated with a cell-penetrating peptide containing the cytoplasmic region of syndecan-4, myoblast fusion and thus myotube formation was blocked, both in normal cells and in syndecan-4 knock down cells. Altogether this suggests that the cytoplasmic domain of syndecan-4 is important in regulation of myogenesis. The internalization of syndecan-4 from the plasma membrane during muscle differentiation and the nuclear localization of syndecan-4 in differentiated muscle cells may be part of this regulation, and is a novel aspect of syndecan biology which merits further studies.

Bidragsytere

Sissel Beate Rønning

  • Tilknyttet:
    Forfatter
    ved Råvare og prosess ved NOFIMA

Cathrine Rein Carlson

  • Tilknyttet:
    Forfatter
    ved Institutt for eksperimentell medisinsk forskning ved Universitetet i Oslo
  • Tilknyttet:
    Forfatter
    ved Inst. eksperimentell med. forsk, Ullevål ved Oslo universitetssykehus HF
  • Tilknyttet:
    Forfatter
    ved K.G. Jebsen senter for hjerteforskning-part UiO ved Universitetet i Oslo

Espen Stang

  • Tilknyttet:
    Forfatter
    ved Avdeling for patologi ved Oslo universitetssykehus HF

Svein Olav Kolset

  • Tilknyttet:
    Forfatter
    ved Molekylær og klinisk glykobiologi ved Universitetet i Oslo

Kristin Hollung

  • Tilknyttet:
    Forfatter
    ved Mat og helse ved NOFIMA
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