Cristin-resultat-ID: 1248893
Sist endret: 11. januar 2016, 12:38
NVI-rapporteringsår: 2015
Resultat
Vitenskapelig artikkel
2015

Methanol-based cadaverine production by genetically engineered Bacillus methanolicus strains

Bidragsytere:
  • Ingemar Nærdal
  • Johannes Pfeifenschneider
  • Trygve Brautaset og
  • Volker F. Wendisch

Tidsskrift

Microbial Biotechnology
ISSN 1751-7907
e-ISSN 1751-7915
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2015
Volum: 8
Hefte: 2
Sider: 342 - 350
Open Access

Importkilder

Scopus-ID: 2-s2.0-84923418693

Beskrivelse Beskrivelse

Tittel

Methanol-based cadaverine production by genetically engineered Bacillus methanolicus strains

Sammendrag

Methanol is regarded as an attractive substrate for biotechnological production of value-added bulk products, such as amino acids and polyamines. In the present study, the methylotrophic and thermophilic bacterium Bacillus methanolicus was engineered into a microbial cell factory for the production of the platform chemical 1,5-diaminopentane (cadaverine) from methanol. This was achieved by the heterologous expression of the Escherichia coli genes cadA and ldcC encoding two different lysine decarboxylase enzymes, and by increasing the overall L-lysine production levels in this host. Both CadA and LdcC were functional in B. methanolicus cultivated at 50°C and expression of cadA resulted in cadaverine production levels up to 500 mg l−1 during shake flask conditions. A volume-corrected concentration of 11.3 g l−1 of cadaverine was obtained by high-cell density fed-batch methanol fermentation. Our results demonstrated that efficient conversion of L-lysine into cadaverine presumably has severe effects on feedback regulation of the L-lysine biosynthetic pathway in B. methanolicus. By also investigating the cadaverine tolerance level, B. methanolicus proved to be an exciting alternative host and comparable to the well-known bacterial hosts E. coli and Corynebacterium glutamicum. This study represents the first demonstration of microbial production of cadaverine from methanol.

Bidragsytere

Ingemar Nærdal

  • Tilknyttet:
    Forfatter
    ved Bioteknologi og nanomedisin ved SINTEF AS

Johannes Pfeifenschneider

  • Tilknyttet:
    Forfatter
    ved Universität Bielefeld

Trygve Brautaset

  • Tilknyttet:
    Forfatter
    ved Institutt for bioteknologi og matvitenskap ved Norges teknisk-naturvitenskapelige universitet
  • Tilknyttet:
    Forfatter
    ved Bioteknologi og nanomedisin ved SINTEF AS

Volker F. Wendisch

  • Tilknyttet:
    Forfatter
    ved Universität Bielefeld
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