Cristin-resultat-ID: 1272143
Sist endret: 24. september 2015, 20:19
Resultat
Vitenskapelig artikkel
2009

Comparison of HPV detection technologies: Hybrid Capture 2, PreTect (TM) HPV-Proofer and analysis of HPV DNA viral load in HPV16, HPV18 and HPV33 E6/E7 mRNA positive specimens

Bidragsytere:
  • Liv Furuberg

Tidsskrift

Journal of Virological Methods
ISSN 0166-0934
e-ISSN 1879-0984
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2009
Volum: 155
Hefte: 1
Sider: 61 - 66

Importkilder

Scopus-ID: 2-s2.0-57649128515
SINTEF AS-ID: S14602
Isi-ID: 000262573400009

Beskrivelse Beskrivelse

Tittel

Comparison of HPV detection technologies: Hybrid Capture 2, PreTect (TM) HPV-Proofer and analysis of HPV DNA viral load in HPV16, HPV18 and HPV33 E6/E7 mRNA positive specimens

Sammendrag

Human papillomavirus (HPV) testing using molecular methods in liquid based cytology (LBC) specimens may be useful as an adjunct to cervical screening by cytology. We compared the positivity rate of the commercially available HPV DNA method hybrid capture 2 (hc2) and the commercially available E6/E7 mRNA method PreTeCt (TM) HPV-Proofer in cytological specimens (n = 299).LBC specimens collected (n = 299) represented the following cervical cytological disease categories: Normal (n = 60), borderline nuclear abnormalities (BNA) (n = 34), CIN1 (n = 121), CIN2 (n = 60), CIN3 (n = 24). Overall, 69% (205/299) of the cases were positive by hc2 and 38% (112/299) of the cases were positive by PreTect (TM) HPV-Proofer. Concordance rates between the two tests were highest in the high-grade cytology cases (CIN2: 67% and CIN3: 83%) and the normal cytology cases (88%) and lowest in the BNA and CIN1 categories (56% and 52%). HPV DNA viral load analyses were carried out on HPV16 (n = 55), HPV18 (n = 9) and HPV33 (n = 13) samples that were positive by PreTect (TM) HPV-Proofer. The sensitivity and specificity of PreTeCt (TM) HPV-Proofer and the hc2 DNA test for the detection of high-grade cytology (i.e. CIN2+) were 71.4% and 75.8% vs 100% and 43.7%, respectively. The relatively low detection rate observed by PreTect (TM) HPV-Proofer in the whole range of cytological positive cases, combined with a relatively higher specificity and PPV. suggests that PreTeCt (TM) HPV-Proofer may be more useful than hc2 for triage and in predicting high-grade disease.

Bidragsytere

Liv Furuberg

  • Tilknyttet:
    Forfatter
    ved Smart Sensors and Microsystems ved SINTEF AS
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