Cristin-resultat-ID: 133223
Sist endret: 21. januar 2015 15:27
Resultat
Poster
2010

TNF-alpha is involved in the release of the acute phase protein PTX3 in lung co-cultures exposed to particulate matter from wood combustion and traffic

Bidragsytere:
  • Jan Inge Herseth
  • Vivi Volden
  • Per Schwarze og
  • Anette Kocbach

Konferanse

Tittel: Inflammation 2010

Sted:

Luxemburg
Utbredelsesområde: Internasjonalt
Årstall: 2010
Dato fra: 27. januar 2010
Dato til: 30. januar 2010

Arrangør:

Recherches Scientifiques Luxembourg

Om resultatet

Poster
Publiseringsår: 2010
Fagfellevurdert

Beskrivelse Beskrivelse

Tittel

TNF-alpha is involved in the release of the acute phase protein PTX3 in lung co-cultures exposed to particulate matter from wood combustion and traffic

Sammendrag

Session 2.2: Cytokines in health and disease. TNF-alpha is involved in the release of the acute phase protein PTX3 in lung co-cultures exposed to particulate matter from wood combustion and traffic. Jan I Herseth 1, 2, Vivi Volden 2, Per E Schwarze 1, Anette Kocbach Bølling 1 1.Division of Environmental Medicine, Norwegian Institute of Public Health, P. O. Box 4404 Nydalen, N-0403 Oslo, Norway, (Per.Schwarze@fhi.no, Anette.Kocbach@fhi.no), 2.Faculty of Health Sciences, Oslo University College, P.O. Box 4 St. Olavs plass, N- 0130 Oslo, Norway, (Jan-Inge.Herseth@hf.hio.no, Vivi.Volden@hf.hio.no) Exposure to ambient particulate matter has been associated with cardiopulmonary morbidity and mortality, and inflammation is thought to play a role in the pulmonary and cardiovascular effects induced by particles. Long pentraxin 3 (PTX3) is a newly discovered acute phase protein produced by cells involved in innate immunity as well as by bronchial and alveolar epithelial cells. Through interaction with for instance pathogens, growth factors and the complement factor C1q, PTX3 may be involved in tuning of the inflammatory response. It has also been suggested as a new marker of innate immunity and inflammation, reflecting tissue and vascular bed responses. The pro-inflammatory cytokines IL-1 and TNF-alpha have both been found to induce release of PTX3. In this study, we used a contact co-culture of monocytic and pneumocytic cell lines to determine the release of PTX3 induced by wood smoke particles (WSP) and different types of traffic derived particles (TDP) after 12, 40 and 64 h of exposure to 40 micrograms/cm2. In addition, anti-TNF-alpha and IL-1 receptor antagonist were used to investigate the influence of these two pro-inflammatory cytokines on the release of PTX3. All particle samples induced a significantly increased release of PTX3 from the co-culture after 40 and 64 h of exposure, but TDP were generally more potent inducers of PTX3. TNF-alpha, but not IL-1, was involved in the release of PTX3 induced by WSP, whereas both these cytokines seemed to be involved in the PTX3 release induced by TDP. The role of PTX3 in particle induced inflammation has not been studied in any detail, and this is the first study investigating the release of PTX3 induced by ambient particulate matter in an in vitro model system. Although the role of PTX3 in particle toxicology needs further elaboration PTX3 may become an interesting marker for particle-induced inflammation.

Bidragsytere

Jan Inge Herseth

  • Tilknyttet:
    Forfatter
    ved Institutt for naturvitenskapelige helsefag ved OsloMet - storbyuniversitetet

Vivi Volden

  • Tilknyttet:
    Forfatter
    ved Institutt for naturvitenskapelige helsefag ved OsloMet - storbyuniversitetet

Per Everhard Schwarze

Bidragsyterens navn vises på dette resultatet som Per Schwarze
  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet

Anette Kocbach

  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet
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