Cristin-resultat-ID: 1367981
Sist endret: 8. oktober 2020, 15:48
NVI-rapporteringsår: 2016
Resultat
Vitenskapelig artikkel
2016

Deciphering the recent phylogenetic expansion of the originally deeply rooted Mycobacterium tuberculosis lineage 7

Bidragsytere:
  • Solomon Abebe Yimer
  • Amine Namouchi
  • Ephrem Debebe Zegeye
  • Carol J C Holm-Hansen
  • Gunnstein Norheim
  • Markos Abebe
  • mfl.

Tidsskrift

BMC Evolutionary Biology
ISSN 1471-2148
e-ISSN 1471-2148
NVI-nivå 2

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2016
Publisert online: 2016
Volum: 16:146
Sider: 1 - 10
Open Access

Importkilder

Scopus-ID: 2-s2.0-84976554455

Beskrivelse Beskrivelse

Tittel

Deciphering the recent phylogenetic expansion of the originally deeply rooted Mycobacterium tuberculosis lineage 7

Sammendrag

Background: A deeply rooted phylogenetic lineage of Mycobacterium tuberculosis (M. tuberculosis) termed lineage 7 was discovered in Ethiopia. Whole genome sequencing of 30 lineage 7 strains from patients in Ethiopia was performed. Intra-lineage genome variation was defined and unique characteristics identified with a focus on genes involved in DNA repair, recombination and replication (3R genes). Results: More than 800 mutations specific to M. tuberculosis lineage 7 strains were identified. The proportion of non-synonymous single nucleotide polymorphisms (nsSNPs) in 3R genes was higher after the recent expansion of M. tuberculosis lineage 7 strain started. The proportion of nsSNPs in genes involved in inorganic ion transport and metabolism was significantly higher before the expansion began. A total of 22346 bp deletions were observed. Lineage 7 strains also exhibited a high number of mutations in genes involved in carbohydrate transport and metabolism, transcription, energy production and conversion. Conclusions: We have identified unique genomic signatures of the lineage 7 strains. The high frequency of nsSNP in 3R genes after the phylogenetic expansion may have contributed to recent variability and adaptation. The abundance of mutations in genes involved in inorganic ion transport and metabolism before the expansion period may indicate an adaptive response of lineage 7 strains to enable survival, potentially under environmental stress exposure. As lineage 7 strains originally were phylogenetically deeply rooted, this may indicate fundamental adaptive genomic pathways affecting the fitness of M. tuberculosis as a species.

Bidragsytere

Aktiv cristin-person

Solomon Abebe Yimer

  • Tilknyttet:
    Forfatter
    ved Avdeling for mikrobiologi ved Oslo universitetssykehus HF
  • Tilknyttet:
    Forfatter
    ved Avdeling for samfunnsmedisin og global helse ved Universitetet i Oslo
Aktiv cristin-person

Amine Namouchi

  • Tilknyttet:
    Forfatter
    ved Avdeling for mikrobiologi ved Oslo universitetssykehus HF

Ephrem Debebe Zegeye

  • Tilknyttet:
    Forfatter
    ved NORCE Miljø ved NORCE Norwegian Research Centre AS

Carol Joanne Church Holm-Hansen

Bidragsyterens navn vises på dette resultatet som Carol J C Holm-Hansen
  • Tilknyttet:
    Forfatter
    ved Avdeling for metodeutvikling og analyse ved Folkehelseinstituttet

Gunnstein Norheim

  • Tilknyttet:
    Forfatter
    ved Avdeling for bakteriologi ved Folkehelseinstituttet
1 - 5 av 8 | Neste | Siste »