Cristin-resultat-ID: 1394645
Sist endret: 17. april 2018 11:50
NVI-rapporteringsår: 2016
Resultat
Vitenskapelig artikkel
2016

The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges

Bidragsytere:
  • Jon Thomassen Hestetun
  • Håkon Dahle
  • Steffen Leth Jørgensen
  • Bernt Rydland Olsen og
  • Hans Tore Rapp

Tidsskrift

Frontiers in Microbiology
ISSN 1664-302X
e-ISSN 1664-302X
NVI-nivå 2

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2016
Publisert online: 2016
Volum: 7:1781
Sider: 1 - 14
Open Access

Importkilder

Scopus-ID: 2-s2.0-85006790618

Finansiering

  • Artsdatabanken

    • Prosjektkode: 70184219
  • Andre

    • Prosjektkode: Det norske vitenskapsakademi
  • Klima- og miljødepartementet

  • Norges forskningsråd

    • Prosjektkode: 179560

Beskrivelse Beskrivelse

Tittel

The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges

Sammendrag

As shown by recent studies, filter-feeding sponges are known to host a wide variety of microorganisms. However, the microbial community of the non-filtering carnivorous sponges (Porifera, Cladorhizidae) has been the subject of less scrutiny. Here, we present the results from a comparative study of the methanotrophic carnivorous sponge Cladorhiza methanophila from a mud volcano-rich area at the Barbados Accretionary Prism (BAP), and five carnivorous species from the Jan Mayen Vent Field (JMVF) at the Arctic Mid-Ocean Ridge. Results from 16S rRNA microbiome data indicate the presence of a diverse assemblage of associated microorganisms in carnivorous sponges mainly from the Gamma- and Alphaproteobacteria, Flavobacteriaceae and Thaumarchaeota. While the abundance of particular groups varied throughout the dataset, we found interesting similarities to previous microbiome results from non-carnivorous deep sea sponges, suggesting that the carnivorous sponges share characteristics of a previously hypothesized putative deep-sea sponge microbial community. Chemolithoautotrophic symbiosis was confirmed for C. methanophila through a microbial community with a high abundance of Methylococcales and very light isotopic δ13C and δ15N ratios (-60 to -66‰/3.5 to 5.2‰) compared to the other cladorhizid species (-22 to -24‰/8.5-10.5‰). We provide evidence for the presence of putative sulfur-oxidizing Gammaproteobacteria in the arctic cladorhizids; however, δ13C and δ15N signatures did not provide evidence for significant chemoautotrophic symbiosis in this case, and the slightly higher abundance of cladorhizids at the JMVF site compared to the nearby deep sea likely stem from an increased abundance of prey rather than a more direct vent association. The phylogenetic position of C. methanophila in relation to other carnivorous sponges was established using a three-gene phylogenetic analysis, and it was found to be closely related to other non-methanotrophic Cladorhiza species with a similar morphology included in the dataset, suggesting a recent origin for methanotrophy in this species. C. methanophila remains the only known carnivorous sponge with a strong, chemolithoautotrophic symbiont association, and methanotrophic symbiosis does not seem to be a widespread property within the Cladorhizidae.

Bidragsytere

Jon Thomassen Hestetun

  • Tilknyttet:
    Forfatter
    ved SFF - Senter for geobiologi ved Universitetet i Bergen
  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap ved Universitetet i Bergen

Håkon Dahle

  • Tilknyttet:
    Forfatter
    ved SFF - Senter for geobiologi ved Universitetet i Bergen

Steffen Leth Jørgensen

  • Tilknyttet:
    Forfatter
    ved SFF - Senter for geobiologi ved Universitetet i Bergen

Bernt Rydland Olsen

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap ved Universitetet i Bergen
  • Tilknyttet:
    Forfatter
    ved SFF - Senter for geobiologi ved Universitetet i Bergen

Hans Tore Rapp

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap ved Universitetet i Bergen
  • Tilknyttet:
    Forfatter
    ved Uni Research Miljø ved NORCE Norwegian Research Centre AS
  • Tilknyttet:
    Forfatter
    ved SFF - Senter for geobiologi ved Universitetet i Bergen
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