Cristin-resultat-ID: 1402823
Sist endret: 13. februar 2017 15:56
NVI-rapporteringsår: 2016
Resultat
Vitenskapelig artikkel
2016

In vitro and in vivo comparison of transport media for detecting nasopharyngeal carriage of Streptococcus pneumoniae

Bidragsytere:
  • Anneke Steens
  • Natacha Alexandra da Fonseca Milhano
  • Ingeborg Sundsvalen Aaberge og
  • DIDRIK FRIMANN VESTRHEIM

Tidsskrift

PeerJ
ISSN 2167-8359
e-ISSN 2167-8359
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2016
Volum: 4
Artikkelnummer: e2449
Open Access

Importkilder

Scopus-ID: 2-s2.0-84991373138

Beskrivelse Beskrivelse

Tittel

In vitro and in vivo comparison of transport media for detecting nasopharyngeal carriage of Streptococcus pneumoniae

Sammendrag

Background. As a standard method for pneumococcal carriage studies, the World Health Organization recommends nasopharyngeal swabs be transported and stored at cool temperatures in a medium containing skim-milk, tryptone, glucose and glycerol (STGG). An enrichment broth used for transport at room temperature in three carriage studies performed in Norway may have a higher sensitivity than STGG. We therefore compared the media in vitro and in vivo. Methods. For the in vitro component, three strains (serotype 4, 19F and 3) were suspended in STGG and enrichment broth. Recovery was compared using latex agglutination, quantification of bacterial loads by real-time PCR of the lytA gene, and counting colonies from incubated plates. For the in vivo comparison, paired swabs were obtained from 100 children and transported in STGG at cool temperatures or in enrichment broth at room temperature. Carriage was identified by latex agglutination and confirmed by Quellung reaction. Results. In vitro, the cycle threshold values obtained by PCR did not differ between the two media (p = 0.853) and no clear difference in colony counts was apparent after incubation (p = 0.593). In vivo, pneurnococci were recovered in 46% of swabs transported in STGG and 51% of those transported in enrichment broth (Kappa statistic 0.90, p = 0.063). Discussion. Overall, no statistical differences in sensitivity were found between STGG and enrichment broth. Nevertheless, some serotype differences were observed and STGG appeared slightly less sensitive than enrichment broth for detection of nasopharyngeal carriage of pneumococci by culturing. We recommend the continued use of STGG for transport and storage of nasopharyngeal swabs in pneumococcal carriage studies for the benefit of comparability between studies and settings, including more resource-limited settings.

Bidragsytere

Anneke Steens

  • Tilknyttet:
    Forfatter
    ved Avdeling for smittevern og vaksine ved Folkehelseinstituttet
  • Tilknyttet:
    Forfatter
    ved Det medisinske fakultet ved Universitetet i Oslo

Natacha Alexandra da Fonseca Milhano

  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet
  • Tilknyttet:
    Forfatter
    ved European Centre for Disease Prevention and Control

Ingeborg Aase S. Aaberge

Bidragsyterens navn vises på dette resultatet som Ingeborg Sundsvalen Aaberge
  • Tilknyttet:
    Forfatter
    ved Smittevern, miljø og helse ved Folkehelseinstituttet

Didrik Frimann Vestrheim

Bidragsyterens navn vises på dette resultatet som DIDRIK FRIMANN VESTRHEIM
  • Tilknyttet:
    Forfatter
    ved Avdeling for smittevern og vaksine ved Folkehelseinstituttet
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