Cristin-resultat-ID: 1453097
Sist endret: 2. mars 2017, 09:52
NVI-rapporteringsår: 2016
Resultat
Vitenskapelig artikkel
2016

New Deferoxamine Glycoconjugates Produced upon Overexpression of Pathway-Specific Regulatory Gene in the Marine Sponge-Derived Streptomyces albus PVA94-07

Bidragsytere:
  • Olga Sekurova
  • Ignacio Pérez-Victoria
  • Jesús Martin
  • Kristin Fløgstad Degnes
  • Håvard Sletta
  • Fernando Reyes
  • mfl.

Tidsskrift

Molecules
ISSN 1431-5157
e-ISSN 1420-3049
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2016
Volum: 21
Hefte: 9:1131
Open Access

Importkilder

Scopus-ID: 2-s2.0-84987896851

Beskrivelse Beskrivelse

Tittel

New Deferoxamine Glycoconjugates Produced upon Overexpression of Pathway-Specific Regulatory Gene in the Marine Sponge-Derived Streptomyces albus PVA94-07

Sammendrag

Activation of silent biosynthetic gene clusters in Streptomyces bacteria via overexpression of cluster-specific regulatory genes is a promising strategy for the discovery of novel bioactive secondary metabolites. This approach was used in an attempt to activate a cryptic gene cluster in a marine sponge-derived Streptomyces albus PVA94-07 presumably governing the biosynthesis of peptide-based secondary metabolites. While no new peptide-based metabolites were detected in the recombinant strain, it was shown to produce at least four new analogues of deferoxamine with additional acyl and sugar moieties, for which chemical structures were fully elucidated. Biological activity tests of two of the new deferoxamine analogues revealed weak activity against Escherichia coli. The gene knockout experiment in the gene cluster targeted for activation, as well as overexpression of certain genes from this cluster did not have an effect on the production of these compounds by the strain overexpressing the regulator. It seems plausible that the production of such compounds is a response to stress imposed by the production of an as-yet unidentified metabolite specified by the cryptic cluster.

Bidragsytere

Olga Sekurova

  • Tilknyttet:
    Forfatter
    ved Universität Wien

Ignacio Pérez-Victoria

  • Tilknyttet:
    Forfatter
    ved Consejo Superior de Investigaciones Cientificas

Jesús Martin

  • Tilknyttet:
    Forfatter
    ved Consejo Superior de Investigaciones Cientificas

Kristin Fløgstad Degnes

  • Tilknyttet:
    Forfatter
    ved Bioteknologi og nanomedisin ved SINTEF AS

Håvard Sletta

  • Tilknyttet:
    Forfatter
    ved Bioteknologi og nanomedisin ved SINTEF AS
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