Cristin-resultat-ID: 1458766
Sist endret: 9. januar 2018, 09:24
NVI-rapporteringsår: 2017
Resultat
Vitenskapelig artikkel
2017

L-lysine production by Bacillus methanolicus: Genome-based mutational analysis and L-lysine secretion engineering

Bidragsytere:
  • Ingemar Nærdal
  • Roman Netzer
  • Marta Irla
  • Anne Krog
  • Tonje Marita Bjerkan Heggset
  • Volker F. Wendisch
  • mfl.

Tidsskrift

Journal of Biotechnology
ISSN 0168-1656
e-ISSN 1873-4863
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2017
Volum: 244
Sider: 25 - 33
Open Access

Importkilder

Scopus-ID: 2-s2.0-85011649745

Beskrivelse Beskrivelse

Tittel

L-lysine production by Bacillus methanolicus: Genome-based mutational analysis and L-lysine secretion engineering

Sammendrag

Bacillus methanolicus is a methylotrophic bacterium with an increasing interest in academic research and for biotechnological applications. This bacterium was previously applied for methanol-based production of l-glutamate, l-lysine and the five-carbon diamine cadaverine by wild type, classical mutant and recombinant strains. The genomes of two different l-lysine secreting B. methanolicus classical mutant strains, NOA2#13A52-8A66 and M168-20, were sequenced. We focused on mutational mapping in genes present in l-lysine and other relevant amino acid biosynthetic pathways, as well as in the primary cell metabolism important for precursor supply. In addition to mutations in the aspartate pathway genes dapG, lysA and hom-1, new mutational target genes like alr, proA, proB1, leuC, odhA and pdhD were identified. Surprisingly, no mutations were found in the putative l-lysine transporter gene lysEMGA3. Inspection of the wild type B. methanolicus strain PB1 genome sequence identified two homologous putative l-lysine transporter genes, lysEPB1 and lysE2PB1. The biological role of these putative l-lysine transporter genes, together with the heterologous l-lysine exporter gene lysECg from Corynebacterium glutamicum, were therefore investigated. Our results demonstrated that the titer of secreted l-lysine in B. methanolicus was significantly increased by overexpression of lysECg while overexpression of lysEMGA3, lysEPB1 and lysE2PB1 had no measurable effect.

Bidragsytere

Ingemar Nærdal

  • Tilknyttet:
    Forfatter
    ved Bioteknologi og nanomedisin ved SINTEF AS

Roman Netzer

  • Tilknyttet:
    Forfatter
    ved Bioteknologi og nanomedisin ved SINTEF AS

Marta Irla

  • Tilknyttet:
    Forfatter
    ved Universität Bielefeld

Anne Krog

  • Tilknyttet:
    Forfatter
    ved Bioteknologi og nanomedisin ved SINTEF AS
Aktiv cristin-person

Tonje Marita Bjerkan Heggeset

Bidragsyterens navn vises på dette resultatet som Tonje Marita Bjerkan Heggset
  • Tilknyttet:
    Forfatter
    ved Bioteknologi og nanomedisin ved SINTEF AS
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