Cristin-resultat-ID: 1473070
Sist endret: 8. oktober 2020, 15:43
NVI-rapporteringsår: 2017
Resultat
Vitenskapelig artikkel
2017

Comparative proteomic analysis of Mycobacterium tuberculosis lineage 7 and lineage 4 strains reveals differentially abundant proteins linked to slow growth and virulence

Bidragsytere:
  • Solomon Abebe Yimer
  • Alemayehu Godana Birhanu
  • Shewit Kalayou
  • Tahira Riaz
  • Ephrem Debebe Zegeye
  • Getachew Tesfaye Beyene
  • mfl.

Tidsskrift

Frontiers in Microbiology
ISSN 1664-302X
e-ISSN 1664-302X
NVI-nivå 2

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2017
Volum: 8:795
Sider: 1 - 14
Open Access

Importkilder

Scopus-ID: 2-s2.0-85019763070

Beskrivelse Beskrivelse

Tittel

Comparative proteomic analysis of Mycobacterium tuberculosis lineage 7 and lineage 4 strains reveals differentially abundant proteins linked to slow growth and virulence

Sammendrag

In order to decipher the nature of the slowly growing Mycobacterium tuberculosis (M.tuberculosis) lineage 7, the differentially abundant proteins in strains ofM. tuberculosis lineage 7 and lineage 4 were defined. Comparative proteomic analysis by mass spectrometry was employed to identify, quantitate and compare the protein profiles of strains from the two M. tuberculosis lineages. Label-free peptide quantification of Whole cells from M. tuberculosis lineage 7 and 4 yielded the identification of 2825 and 2541 proteins, respectively. A combined total of 2867 protein groups covering 71% of the predicted M. tuberculosis proteome were identified. The abundance of 125 proteins in M. tuberculosis lineage 7 and 4 strains was significantly altered. Notably, the analysis showed that a number of M. tuberculosis proteins involved in growth and virulence were less abundant in lineage 7 strains compared to lineage 4. Five ABC transporter proteins, three phosphate binding proteins essential for inorganic phosphate uptake, and six components of the type 7 secretion system ESX-3 involved in iron acquisition were less abundant in M. tuberculosis lineage 7. This proteogenomic analysis provided an insight into the lineage 7-specific protein profile which may provide clues to understanding the differential properties of lineage 7 strains in terms of slow growth, survival fitness, and pathogenesis.

Bidragsytere

Aktiv cristin-person

Solomon Abebe Yimer

  • Tilknyttet:
    Forfatter
    ved Avdeling for mikrobiologi ved Universitetet i Oslo
  • Tilknyttet:
    Forfatter
    ved Avdeling for mikrobiologi ved Oslo universitetssykehus HF

Alemayehu Godana Birhanu

  • Tilknyttet:
    Forfatter
    ved Addis Ababa University
  • Tilknyttet:
    Forfatter
    ved Avdeling for mikrobiologi ved Universitetet i Oslo

Shewit Kalayou Teklehaimanot

Bidragsyterens navn vises på dette resultatet som Shewit Kalayou
  • Tilknyttet:
    Forfatter
    ved Avdeling for mikrobiologi ved Oslo universitetssykehus HF

Tahira Riaz

  • Tilknyttet:
    Forfatter
    ved Avdeling for mikrobiologi ved Universitetet i Oslo

Ephrem Debebe Zegeye

  • Tilknyttet:
    Forfatter
    ved NORCE Miljø ved NORCE Norwegian Research Centre AS
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