Cristin-resultat-ID: 1478075
Sist endret: 8. mars 2018 13:57
NVI-rapporteringsår: 2017
Resultat
Vitenskapelig artikkel
2017

Deep sequencing analysis of tick-borne encephalitis virus from questing ticks at natural foci reveals similarities between quasispecies pools of the virus

Bidragsytere:
  • Naveed Asghar
  • John Pettersson
  • Patrik Dinnetz
  • Åshild Kristine Andreassen og
  • Magnus Johansson

Tidsskrift

Journal of General Virology
ISSN 0022-1317
e-ISSN 1465-2099
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2017
Volum: 98
Hefte: 3
Sider: 413 - 421
Open Access

Importkilder

Scopus-ID: 2-s2.0-85016952345

Finansiering

  • Helse- og omsorgsdepartementet
    Prosjektkode: B1710

Beskrivelse Beskrivelse

Tittel

Deep sequencing analysis of tick-borne encephalitis virus from questing ticks at natural foci reveals similarities between quasispecies pools of the virus

Sammendrag

Every year, tick-borne encephalitis virus (TBEV) causes severe central nervous system infection in 10 000 to 15 000 people in Europe and Asia. TBEV is maintained in the environment by an enzootic cycle that requires a tick vector and a vertebrate host, and the adaptation of TBEV to vertebrate and invertebrate environments is essential for TBEV persistence in nature. This adaptation is facilitated by the error-prone nature of the virus's RNA-dependent RNA polymerase, which generates genetically distinct virus variants called quasispecies. TBEV shows a focal geographical distribution pattern where each focus represents a TBEV hotspot. Here, we sequenced and characterized two TBEV genomes, JP-296 and JP-554, from questing Ixodes ricinus ticks at a TBEV focus in central Sweden. Phylogenetic analysis showed geographical clustering among the newly sequenced strains and three previously sequenced Scandinavian strains, Toro-2003, Saringe-2009 and Mandal-2009, which originated from the same ancestor. Among these five Scandinavian TBEV strains, only Mandal-2009 showed a large deletion within the 3' non-coding region (NCR), similar to the highly virulent TBEV strain Hypr. Deep sequencing of JP-296, JP-554 and Mandal-2009 revealed significantly high quasispecies diversity for JP-296 and JP-554, with intact 3' NCRs, compared to the low diversity in Mandal-2009, with a truncated 3' NCR. Single-nucleotide polymorphism analysis showed that 40% of the single-nucleotide polymorphisms were common between quasispecies populations of JP-296 and JP-554, indicating a putative mechanism for how TBEV persists and is maintained within its natural foci.

Bidragsytere

Naveed Asghar

  • Tilknyttet:
    Forfatter
    ved Södertörns högskola
  • Tilknyttet:
    Forfatter
    ved Örebro universitet

John Pettersson

  • Tilknyttet:
    Forfatter
    ved Uppsala universitet
  • Tilknyttet:
    Forfatter
    ved Statens veterinärmedicinska anstalt
  • Tilknyttet:
    Forfatter
    ved Avdeling for infeksjonsepidemiologi og modellering ved Folkehelseinstituttet

Patrik Dinnetz

  • Tilknyttet:
    Forfatter
    ved Södertörns högskola

Åshild Kristine Andreassen

  • Tilknyttet:
    Forfatter
    ved Avdeling for virologi ved Folkehelseinstituttet

Magnus Johansson

  • Tilknyttet:
    Forfatter
    ved Örebro universitet
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