Cristin-resultat-ID: 1478937
Sist endret: 17. oktober 2017, 11:02
NVI-rapporteringsår: 2017
Resultat
Vitenskapelig artikkel
2017

Extraction, Solubility and Antimicrobial Activity of (-) Usnic Acid in Ethanol, a Pharmaceutically Relevant Solvent

Bidragsytere:
  • Rafi Ahmad
  • Paulina Borowiec
  • Anne Bergljot Falck-Ytter og
  • Knut Olav Strætkvern

Tidsskrift

Natural Product Communications
ISSN 1934-578X
e-ISSN 1555-9475
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2017
Publisert online: 2017
Trykket: 2017
Volum: 12
Hefte: 7
Sider: 1101 - 1104
Open Access

Importkilder

Scopus-ID: 2-s2.0-85026466845

Beskrivelse Beskrivelse

Tittel

Extraction, Solubility and Antimicrobial Activity of (-) Usnic Acid in Ethanol, a Pharmaceutically Relevant Solvent

Sammendrag

Usnic acid (UA) is the most studied bioactive secondary metabolite from lichens. Recently, UA research has focused on its antibacterial activity. However, increased application in healthcare products of UA is hampered by low solubility in aqueous solutions. Here, we report ethanol both as an alternative extraction solvent to acetone and as a pharmaceutically relevant solvent for future formulations. Dried samples of the abundant reindeer lichen (Cladonia stellaris) were extracted in a 23 factorial design using either ethanol or acetone, varying both time and solvent-to-dry mass ratio. In both extracts, UA was the predominant compound as shown by two-dimensional thin-layer chromatography (TLC). In a disc diffusion assay, both extracts demonstrated similar antibacterial susceptibility towards Gram-positive (Bacillus subtilis and Staphylococcus aureus), while showing little to no activity against Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa). Among a range of pharmaceutically relevant solvents tested, UA was only soluble in ethanol (at concentrations up to 1-2 mg mL-1).

Bidragsytere

Rafi Ahmad

  • Tilknyttet:
    Forfatter
    ved Institutt for bioteknologi ved Høgskolen i Innlandet

Paulina Borowiec

  • Tilknyttet:
    Forfatter
    ved Institutt for bioteknologi ved Høgskolen i Innlandet

Anne Bergljot Falck-Ytter

  • Tilknyttet:
    Forfatter
    ved Institutt for bioteknologi ved Høgskolen i Innlandet

Knut Olav Strætkvern

  • Tilknyttet:
    Forfatter
    ved Institutt for bioteknologi ved Høgskolen i Innlandet
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