Cristin-resultat-ID: 1489061
Sist endret: 15. mars 2018, 15:46
Resultat
Poster
2017

Quinolone resistant Escherichia coli from broiler in Norway - characterization, comparison to human isolates and molecular risk assessment

Bidragsytere:
  • Jannice Schau Slettemeås
  • Madelaine Norström
  • Marianne Sunde
  • Anne Margrete Urdahl
  • Astrid Lousie Wester
  • Karin Lagesen
  • mfl.

Presentasjon

Navn på arrangementet: 7th Symposium on Antimicrobial Resistance in Animals and the Environment
Sted: Braunschweig
Dato fra: 26. juni 2017
Dato til: 28. juni 2017

Om resultatet

Poster
Publiseringsår: 2017

Beskrivelse Beskrivelse

Tittel

Quinolone resistant Escherichia coli from broiler in Norway - characterization, comparison to human isolates and molecular risk assessment

Sammendrag

Background and objectives: Usage of fluoroquinolones (FQ) in Norwegian livestock production is negligible. Historically the occurrence of quinolone resistant Escherichia coli (QREC) in the Norwegian monitoring program for antimicrobial resistance in the veterinary sector (NORM-VET) has been rare. However, in NORM-VET 2014 a selective method was implemented for screening of QREC in broilers and QREC was found in 89.5% of caecal samples and in 70.7% of meat samples. In this study we aim to characterize and compare QREC from broilers and humans in Norway in order to investigate possible links between isolates of the two reservoirs. Material and methods: NORM-VET 2014 isolates from chicken meat (n=47) and caecal samples (n=53) resistant to nalidixic acid (NAL) and/or ciprofloxacin (CIP) were selected for sequencing. Human QREC isolates were collected via the Norwegian surveillance program for antimicrobial resistance (NORM) from urinary tract infections and bacteraemia (n=77), and from screening healthy carriers for QREC (n=23). Data regarding antimicrobial resistance were extracted from the monitoring programs (NORM-VET and NORM) and compared between isolates. The 200 isolates were sequenced using Nextera XT library prep on HiSeq 2500 with Rapid Run. The sequence data were trimmed using Trimmomatic, and assembled using SPAdes. We are currently analysing the sequence data for presence of resistance- and virulence genes, plasmids, MLST and SNP analysis. Results: NORM-VET 2014 resistance data showed that the majority of the broiler isolates exhibited high level resistance to NAL (MICs >128 mg/L), but low level tolerance to CIP (MICs 0.12-0.5). Mutations in the gyrA gene were identified in most isolates, and a few exhibited the plasmid mediated resistance gene qnrS1. Data from the NORM program showed that the human isolates had a different MIC distribution with the largest proportion of isolates exhibiting high level CIP resistance (MICs >0.25), indicative of more mutations in the gyrA/parC genes. However, a considerable proportion of human isolates exhibited a quinolone resistance MIC profile corresponding to the most common profile found among the broiler isolates. Conclusions: Preliminary results indicate that the main mechanism behind quinolone resistance in this study is mutations in gyrA, but plasmid mediated resistance were also identified. Further, the human isolates exhibit high level CIP resistance compared to low tolerance in the broiler isolates.

Bidragsytere

Jannice Schau Slettemeås

  • Tilknyttet:
    Forfatter
    ved Mattrygghet og nye helsetrusler ved Veterinærinstituttet

Madelaine Norström

  • Tilknyttet:
    Forfatter
    ved Epidemiologi ved Veterinærinstituttet

Marianne Sunde

  • Tilknyttet:
    Forfatter
    ved Mattrygghet og nye helsetrusler ved Veterinærinstituttet

Anne Margrete Urdahl

  • Tilknyttet:
    Forfatter
    ved Mattrygghet og nye helsetrusler ved Veterinærinstituttet

Astrid Louise Wester

Bidragsyterens navn vises på dette resultatet som Astrid Lousie Wester
  • Tilknyttet:
    Forfatter
    ved Avdeling for bakteriologi ved Folkehelseinstituttet
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