Cristin-resultat-ID: 1583737
Sist endret: 7. mai 2018, 06:29
Resultat
Vitenskapelig foredrag
2018

Growth-related epigenetic crossroads in fast muscle of Nile tilapia sharing a similar genetic background

Bidragsytere:
  • Tomasz Krzysztof Podgorniak
  • Sven Brockmann og
  • Jorge Manuel de Oliveira Fernandes

Presentasjon

Navn på arrangementet: GIA2018: 5th International Symposium on Genomics in Aquaculture
Sted: Albufeira
Dato fra: 21. mars 2018
Dato til: 23. mars 2018

Om resultatet

Vitenskapelig foredrag
Publiseringsår: 2018

Beskrivelse Beskrivelse

Tittel

Growth-related epigenetic crossroads in fast muscle of Nile tilapia sharing a similar genetic background

Sammendrag

Fish growth rate is one of the crucial factors for a sustainable and profitable aquaculture. Thus, many studies in applied research focused on growth improvement strategies, starting from selective breeding favoring specific gene candidates to optimization of rearing conditions. While artificial selection is based on a priori known genetic differences between slow and fast-growing phenotypes, little is still known on epigenetic mechanisms underlying growth rate differences. In order to get insights into epigenetic mechanisms regulating growth phenotype in tilapia, we used reduced representation bisulfite sequencing (RRBS) to compare the fast muscle methylomes of slow and fast growing fish coming from the same mother. Our hypothesis is that they could explain differences in phenotype patterns even when the parental background is similar. In this study, we compared the methylome of 24 fish (12 large- and 12 small-sized individuals, evenly distributed sex). We found that CpG methylation is related to body weight, but often in a sex-dependent manner. For example, the location of hypermethylated sites in large females were different from hypermethylated sites in large males. Only few CpG sites shared the same methylation patterns between the sexes. Surprisingly, several highly significant differentially methylated cytosines related to fast growth showed the opposite patterns between sexes. Enrichment analysis performed on each set of genes revealed the diversity of biological processes involved in growth regulation and suggests sex-specific pathways. Based on gene ontology terms, we found several connections between cytosine methylation, histone methylation and transcription factors suggesting that epigenetic regulation of growth in Nile tilapia takes place at different levels. Acknowledgements: This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement no 683210) and from the Research Council of Norway under the Toppforsk programme (grant agreement no 250548/F20).

Bidragsytere

Tomasz Podgorniak

Bidragsyterens navn vises på dette resultatet som Tomasz Krzysztof Podgorniak
  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet

Sven Brockmann

  • Tilknyttet:
    Forfatter
Aktiv cristin-person

Jorge Manuel de Oliveira Fernandes

  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet
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