Cristin-resultat-ID: 1584680
Sist endret: 11. juli 2018, 13:45
NVI-rapporteringsår: 2018
Resultat
Vitenskapelig artikkel
2018

Molecular and antigenic characterization of piscine orthoreovirus (PRV) from rainbow trout (oncorhynchus orthoreovirumykiss)

Bidragsytere:
  • Dhamotharan Kannimuthu
  • Niccolò Vendramin
  • Turhan Markussen
  • Øystein Wessel
  • Argelia Cuenca
  • Ingvild Berg Nyman
  • mfl.

Tidsskrift

Viruses
ISSN 1999-4915
e-ISSN 1999-4915
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2018
Publisert online: 2018
Trykket: 2018
Volum: 10
Hefte: 4
Sider: 1 - 16
Artikkelnummer: 170
Open Access

Importkilder

Scopus-ID: 2-s2.0-85045119528

Beskrivelse Beskrivelse

Tittel

Molecular and antigenic characterization of piscine orthoreovirus (PRV) from rainbow trout (oncorhynchus orthoreovirumykiss)

Sammendrag

Piscine orthoreovirus (PRV-1) causes heart and skeletal muscle inflammation (HSMI) in farmed Atlantic salmon (Salmo salar). Recently, a novel PRV (formerly PRV-Om, here called PRV-3), was found in rainbow trout (Oncorhynchus mykiss) with HSMI-like disease. PRV is considered to be an emerging pathogen in farmed salmonids. In this study, molecular and antigenic characterization of PRV-3 was performed. Erythrocytes are the main target cells for PRV, and blood samples that were collected from experimentally challenged fish were used as source of virus. Virus particles were purified by gradient ultracentrifugation and the complete coding sequences of PRV-3 were obtained by Illumina sequencing. When compared to PRV-1, the nucleotide identity of the coding regions was 80.1%, and the amino acid identities of the predicted PRV-3 proteins varied from 96.7% (λ1) to 79.1% (σ3). Phylogenetic analysis showed that PRV-3 belongs to a separate cluster. The region encoding σ3 were sequenced from PRV-3 isolates collected from rainbow trout in Europe. These sequences clustered together, but were distant from PRV-3 that was isolated from rainbow trout in Norway. Bioinformatic analyses of PRV-3 proteins revealed that predicted secondary structures and functional domains were conserved between PRV-3 and PRV-1. Rabbit antisera raised against purified virus or various recombinant virus proteins from PRV-1 all cross-reacted with PRV-3. Our findings indicate that despite different species preferences of the PRV subtypes, several genetic, antigenic, and structural properties are conserved between PRV-1 and-3.

Bidragsytere

Dhamotharan Kannimuthu

  • Tilknyttet:
    Forfatter
    ved Institutt for prekliniske fag og patologi ved Norges miljø- og biovitenskapelige universitet

Niccolò Vendramin

  • Tilknyttet:
    Forfatter
    ved Danmarks Tekniske Universitet

Turhan Markussen

  • Tilknyttet:
    Forfatter
    ved Institutt for prekliniske fag og patologi ved Norges miljø- og biovitenskapelige universitet

Øystein Wessel

  • Tilknyttet:
    Forfatter
    ved Institutt for prekliniske fag og patologi ved Norges miljø- og biovitenskapelige universitet

Argelia Cuenca

  • Tilknyttet:
    Forfatter
    ved Danmarks Tekniske Universitet
1 - 5 av 10 | Neste | Siste »