Cristin-resultat-ID: 1653192
Sist endret: 29. januar 2019, 12:36
NVI-rapporteringsår: 2018
Vitenskapelig artikkel

Evidence of functional Cd94 polymorphism in a free-living house mouse population

  • Linn Emilie Knutsen
  • Erik Dissen
  • Per Christian Sæther
  • Elisabeth Gyllensten Bjørnsen
  • Jaroslav Pialek
  • Anne Storset
  • mfl.


ISSN 0093-7711
e-ISSN 1432-1211
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2018
Publisert online: 2018
Sider: 1 - 13


Scopus-ID: 2-s2.0-85058164690

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Evidence of functional Cd94 polymorphism in a free-living house mouse population


The CD94 receptor, expressed on natural killer (NK) and CD8+ T cells, is known as a relatively non-polymorphic receptor with orthologues in humans, other primates, cattle, and rodents. In the house mouse (Mus musculus), a single allele is highly conserved among laboratory strains, and reports of allelic variation in lab- or wild-living mice are lacking, except for deficiency in one lab strain (DBA/2J). The non-classical MHC-I molecule Qa-1b is the ligand for mouse CD94/NKG2A, presenting alternative non-americ fragment of leader peptides (Qa-1 determinant modifier (Qdm)) from classical MHC-I molecules. Here, we report a novel allele identified in free-living house mice captured in Norway, living among individuals carrying the canonical Cd94 allele. The novel Cd94LocA allele encodes 12 amino acid substitutions in the extracellular lectin-like domain. Flow cytometric analysis of primary NK cells and transfected cells indicates that the substitutions prevent binding of CD94 mAb and Qa-1b/Qdm tetramers. Our data further indicate correlation of Cd94 polymorphism with the two major subspecies of house mice in Europe. Together, these findings suggest that the Cd94LocA/NKG2A heterodimeric receptor is widely expressed among M. musculus subspecies musculus, with ligand-binding properties different from mice of subspecies domesticus, such as the C57BL/6 strain.


Linn Emilie Knutsen

  • Tilknyttet:
    ved Institutt for prekliniske fag og patologi ved Norges miljø- og biovitenskapelige universitet

Erik Dissen

  • Tilknyttet:
    ved NK-celle-gruppen ved Universitetet i Oslo

Per Christian Sæther

  • Tilknyttet:
    ved Anatomi: Immunbiologi ved Universitetet i Oslo

Elisabeth Gyllensten Bjørnsen

  • Tilknyttet:
    ved NK-celle-gruppen ved Universitetet i Oslo

Jaroslav Pialek

  • Tilknyttet:
    ved Institute of Vertebrate Biology ASCR
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