Cristin-resultat-ID: 1663124
Sist endret: 22. januar 2019, 14:57
Resultat
Vitenskapelig foredrag
2018

The mitochondrial epigenome of Nile tilapia(Oreochromis niloticus)

Bidragsytere:
  • Maria Renström
  • Tomasz Krzysztof Podgorniak
  • Paul Whatmore
  • Francesc Piferrer
  • Steinar Daae Johansen
  • Kaja Helvik Skjærven
  • mfl.

Presentasjon

Navn på arrangementet: GIA2018: 5th International Symposium on Genomics in Aquaculture
Dato fra: 21. mars 2018
Dato til: 23. mars 2018

Om resultatet

Vitenskapelig foredrag
Publiseringsår: 2018

Beskrivelse Beskrivelse

Tittel

The mitochondrial epigenome of Nile tilapia(Oreochromis niloticus)

Sammendrag

Nile tilapia (Oreochromis niloticus) is one of the most important and widely farmed aquaculture species. Skeletal muscle is the main contributor to body mass (up to 40% of total weight) and the most valuable component for the farming industry. Growth is closely linked to mitochondrion, since this organelle’s primary function is generation of cellular energy by production of ATP (adenosine triphosphate). The amount of mitochondria per cell varies significantly depending on type of tissue. In fish, white anaerobic muscle, the major part of skeletal muscle, has a low mitochondria content per fiber, whereas red muscle with aerobic metabolism contain a high amount of mitochondria per muscle cell. Mitochondrion has a compact circular genome (16,5 Kb in average), which is related to its bacterial origin. The mitogenome in Nile tilapia is sequenced and is 16,625 bp long. It contains 13 protein-coding genes (involved in oxidative phosphorylation), two rRNA genes, 22 tRNA genes and a D-loop (putative control region). The number and the order of mitochondrial genes in Nile tilapia corresponds to those in the other Cichlids fishes. DNA methylation can influence mitochondrial gene expression by affecting the D-loop region, the main control site for mtDNA transcription and replication. For example, in skeletal muscle, the effect of mitochondrial DNA (mtDNA) methylation could potentially lead to mitophagy, a mechanism of the loss of muscle mitochondria. Epigenetic changes associated with mtDNA in muscle tissues and their potential connection with growth in Nile tilapia are still a question that remains largely unanswered. In this study, we isolated mtDNA from white muscle of 16 full-sib Nile tilapia females and males. Pico methyl-seq library prep kit (Zymo Research, USA) was used for constructing mtDNA libraries for further Whole Genome Bisulfite Sequencing on Illumina MiSeq. Our results suggest that DNA methylation of the mitochondrial genome may regulate epigenetically the expression of several genes that are important for muscle growth in Nile tilapia. These data will contribute to the identification of potential epigenetic markers and mechanisms controlling the growth rate of Nile tilapia, and will provide better understanding of epigenetics as a new layer of information that will be integrated in future breeding selection programs and biotechnology in aquaculture.

Bidragsytere

Maria Renström

  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet

Tomasz Podgorniak

Bidragsyterens navn vises på dette resultatet som Tomasz Krzysztof Podgorniak
  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet

Paul Whatmore

  • Tilknyttet:
    Forfatter
    ved Marin toksikologi ved Havforskningsinstituttet

Francesc Piferrer

  • Tilknyttet:
    Forfatter
    ved Instituto de Ciencias del Mar

Steinar Daae Johansen

  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet
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