Cristin-resultat-ID: 1683152
Sist endret: 16. januar 2020, 09:28
NVI-rapporteringsår: 2019
Resultat
Vitenskapelig artikkel
2019

Influence of nitrogen limitation on lipid accumulation and EPA and DHA content in four marine microalgae for possible use in aquafeed

Bidragsytere:
  • Xinxin Wang
  • Hilde Karoline Fosse
  • Keshuai Li
  • Matilde Skogen Chauton
  • Olav Vadstein og
  • Kjell Inge Reitan

Tidsskrift

Frontiers in Marine Science
ISSN 2296-7745
e-ISSN 2296-7745
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2019
Publisert online: 2019
Trykket: 2019
Open Access

Importkilder

Scopus-ID: 2-s2.0-85065419633

Beskrivelse Beskrivelse

Tittel

Influence of nitrogen limitation on lipid accumulation and EPA and DHA content in four marine microalgae for possible use in aquafeed

Sammendrag

Microalgae are regarded as a promising alternative that can replace fishmeal and fish oil in aquaculture. Under N-limitation, many microalgae species change their carbon storage patterns in favor of neutral lipids (NLs) mainly in the form of triacylglycerol (TAG), but fatty acids in polar lipids (PL) are nutritionally more available for fish than those esterified into NLs. In the present study, the effect of N-limitation on the lipid content and fatty acid profiles in different lipid class of Phaeodactylum tricornutum, Isochrysis aff. galbana clone T-Iso, Rhodomonas baltica and Nannochloropsis oceanica were investigated. The microalgae cells were cultivated by two different methods, batch and semi-continuous culture, to create strong and moderate N-limitation, and this in turn will significantly affect the biomass and lipid productivity. All four species accumulated lipids mainly in the form of TAG in response to strong nitrogen limitation. N. oceanica, however accumulated 51% of the dry weight as lipid in moderate nitrogen limitation and up to 87% of the fatty acid was in TAG. Isochrysis aff. galbana clone T-Iso was the only species where the fraction of polyunsaturated fatty acid (PUFA), especially the fraction of docosahexaenoic acid (DHA) increased with increasing nitrogen limitation. Total lipid productivity showed no increase in batch culture although stronger nitrogen limitation led to lipid accumulation. P. tricornutum had the highest eicosapentaenoic acid (EPA) content, while N. oceanica showed the highest EPA productivity due to the high content of lipid. The highest DHA productivity was found in Isochrysis aff. galbana clone T-Iso from moderate N-limitation mainly due to the high biomass productivity. Based on the results from the current study, N. oceanica and T-Iso are two promising microalgae strains as long-term sustainable source of n-3 long chain -PUFAs under moderate N-limitation. As shown in the present study, increased lipid content in microalgal cells due to strong N-limitation induction may not increase the lipid productivity because biomass production usually is reduced. Therefore, a combination of approaches such as metabolic engineering, conditioning and selection may be needed to further increase the n-3 LC-PUFA productivity without substantial loss of biomass.

Bidragsytere

Xinxin Wang

  • Tilknyttet:
    Forfatter
    ved Institutt for bioteknologi og matvitenskap ved Norges teknisk-naturvitenskapelige universitet
  • Tilknyttet:
    Forfatter
    ved Institutt for biologi ved Norges teknisk-naturvitenskapelige universitet

Hilde Karoline Fosse

  • Tilknyttet:
    Forfatter
    ved Institutt for biologi ved Norges teknisk-naturvitenskapelige universitet

Keshuai Li

  • Tilknyttet:
    Forfatter
    ved Institutt for biologi ved Norges teknisk-naturvitenskapelige universitet

Mathilde Skogen Chauton

Bidragsyterens navn vises på dette resultatet som Matilde Skogen Chauton
  • Tilknyttet:
    Forfatter
    ved Fiskeri og ny biomarin industri ved SINTEF Ocean

Olav Vadstein

  • Tilknyttet:
    Forfatter
    ved Institutt for bioteknologi og matvitenskap ved Norges teknisk-naturvitenskapelige universitet
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