Cristin-resultat-ID: 1708084
Sist endret: 14. august 2019, 12:23
NVI-rapporteringsår: 2019
Resultat
Vitenskapelig artikkel
2019

Genomic and transcriptomic analysis of amoebisc gill disease resistansce in Atlantic salmon(salmo salar L.)

Bidragsytere:
  • Solomon Antwi Boison
  • Bjarne Gjerde
  • Borghild Hillestad
  • Shokouh Makvandi-Nejad og
  • Hooman Moghadam

Tidsskrift

Frontiers in Genetics
ISSN 1664-8021
e-ISSN 1664-8021
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2019
Publisert online: 2019
Volum: 10
Sider: 1 - 15
Artikkelnummer: 68
Open Access

Importkilder

Scopus-ID: 2-s2.0-85065979333

Beskrivelse Beskrivelse

Tittel

Genomic and transcriptomic analysis of amoebisc gill disease resistansce in Atlantic salmon(salmo salar L.)

Sammendrag

Amoebic gill disease (AGD) is one of the most important parasitic diseases of farmed Atlantic salmon. It is a source ofmajor economic loss to the industry and poses significant threats to animal welfare. Previous studies have shown that resistance against this disease has a moderate, heritable genetic component, although the genes and the genetic pathways that contribute to this process have yet to be elucidated. In this study, to identify the genetic mechanisms of AGD resistance, we first investigated the molecular signatures of AGD infection in Atlantic salmon through a challenge model, where we compared the transcriptome profiles of the naïve and infected animals. We then conducted a genome-wide association analysis with 1,333 challenged tested fish to map the AGD resistance genomic regions, supported by the results from the transcriptomic data. Further, we investigated the potential of incorporating gene expression analysis results in genomic prediction to improve prediction accuracy. Our data suggest thousands of genes have modified their expression following infection, with a significant increase in the transcription of genes with functional properties in cell adhesion and a sharp decline in the abundance of various components of the immune system genes. From the genome-wide association analysis, QTL regions on chromosomes ssa04, ssa09, and ssa13 were detected to be linked with AGD resistance. In particular, we found that QTL region on ssa04 harbors members of the cadherin gene family. These genes play a critical role in target recognition and cell adhesion. The QTL region on ssa09 also is associated with another member of the cadherin gene family, protocadherin Fat 4. The associated genetic markers on ssa13 span a large genomic region that includes interleukin-18-binding protein, a gene with function essential in inhibiting the proinflammatory effect of cytokine IL18. Incorporating gene expression information through a weighted genomic relationship matrix approach decreased genomic prediction accuracy and increased bias of prediction. Together, these findings help to improve our breeding programs and animal welfare against AGD and advance our knowledge of the genetic basis of host-pathogen interactions.

Bidragsytere

Solomon Antwi Boison

Bidragsyterens navn vises på dette resultatet som Solomon Antwi Boison
  • Tilknyttet:
    Forfatter
    ved Avl og genetikk ved NOFIMA

Bjarne Gjerde

  • Tilknyttet:
    Forfatter
    ved Avl og genetikk ved NOFIMA

Borghild Hillestad

  • Tilknyttet:
    Forfatter
    ved Salmobreed

Shokouh Makvandi-Nejad

  • Tilknyttet:
    Forfatter
    ved Forskningsgruppe dyrehelse ved Veterinærinstituttet

Hooman Moghadam

  • Tilknyttet:
    Forfatter
    ved Salmobreed
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