Cristin-resultat-ID: 1783214
Sist endret: 14. februar 2020, 14:23
NVI-rapporteringsår: 2019
Resultat
Vitenskapelig artikkel
2019

Genetic determinants of genus-level glycan diversity in a bacterial protein glycosylation system

Bidragsytere:
  • Chris Hadjineophytou
  • Jan Haug Anonsen
  • Nelson Wang
  • Kevin Ma
  • Raimonda Viburiene
  • Åshild Vik
  • mfl.

Tidsskrift

PLoS Genetics
ISSN 1553-7390
e-ISSN 1553-7404
NVI-nivå 2

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2019
Publisert online: 2019
Volum: 15
Hefte: 12
Sider: 1 - 24
Artikkelnummer: e1008532
Open Access

Importkilder

Scopus-ID: 2-s2.0-85077648216

Beskrivelse Beskrivelse

Tittel

Genetic determinants of genus-level glycan diversity in a bacterial protein glycosylation system

Sammendrag

The human pathogens N. gonorrhoeae and N. meningitidis display robust intra- and interstrain glycan diversity associated with their O-linked protein glycosylation (pgl) systems. In an effort to better understand the evolution and function of protein glycosylation operating there, we aimed to determine if other human-restricted, Neisseria species similarly glycosylate proteins and if so, to assess the levels of glycoform diversity. Comparative genomics revealed the conservation of a subset of genes minimally required for O-linked protein glycosylation glycan and established those pgl genes as core genome constituents of the genus. In conjunction with mass spectrometric–based glycan phenotyping, we found that extant glycoform repertoires in N. gonorrhoeae, N. meningitidis and the closely related species N. polysaccharea and N. lactamica reflect the functional replacement of a progenitor glycan biosynthetic pathway. This replacement involved loss of pgl gene components of the primordial pathway coincident with the acquisition of two exogenous glycosyltransferase genes. Critical to this discovery was the identification of a ubiquitous but previously unrecognized glycosyltransferase gene (pglP) that has uniquely undergone parallel but independent pseudogenization in N. gonorrhoeae and N. meningitidis. We suggest that the pseudogenization events are driven by processes of compositional epistasis leading to gene decay. Additionally, we documented instances where inter-species recombination influences pgl gene status and creates discordant genetic interactions due ostensibly to the multi-locus nature of pgl gene networks. In summary, these findings provide a novel perspective on the evolution of protein glycosylation systems and identify phylogenetically informative, genetic differences associated with Neisseria species.

Bidragsytere

Chris Hadjineophytou

  • Tilknyttet:
    Forfatter
    ved Genetikk og evolusjonsbiologi ved Universitetet i Oslo

Jan Haug Anonsen

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap ved Universitetet i Oslo

Nelson Wang

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap ved Universitetet i Oslo

Kevin Ma

  • Tilknyttet:
    Forfatter
    ved Harvard School of Public Health

Raimonda Viburiene

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap ved Universitetet i Oslo
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