Cristin-resultat-ID: 1823588
Sist endret: 11. november 2020, 15:50
NVI-rapporteringsår: 2020
Resultat
Vitenskapelig artikkel
2020

Significant taxon sampling gaps in DNA databases limit the operational use of marine macrofauna metabarcoding

Bidragsytere:
  • Jon Thomassen Hestetun
  • Einar Bye-Ingebrigtsen
  • R Henrik Nilsson
  • Adrian G. Glover
  • Per-Otto Johansen og
  • Thomas Gunnar Dahlgren

Tidsskrift

Marine Biodiversity
ISSN 1867-1616
e-ISSN 1867-1624
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2020
Publisert online: 2020
Volum: 50
Artikkelnummer: 70

Importkilder

Scopus-ID: 2-s2.0-85089437076

Beskrivelse Beskrivelse

Tittel

Significant taxon sampling gaps in DNA databases limit the operational use of marine macrofauna metabarcoding

Sammendrag

Significant effort is spent on monitoring of benthic ecosystems through government funding or indirectly as a cost of business, and metabarcoding of environmental DNA samples has been suggested as a possible complement or alternative to current morphological methods to assess biodiversity. In metabarcoding, a public sequence database is typically used to match barcodes to species identity, but these databases are naturally incomplete. The North Sea oil and gas industry conducts large-scale environmental monitoring programs in one of the most heavily sampled marine areas worldwide and could therefore be considered a “best-case scenario” for macrofaunal metabarcoding. As a test case, we investigated the database coverage of two common metabarcoding markers, mitochondrial COI and the ribosomal rRNA 18S gene, for a complete list of 1802 macrofauna taxa reported from the North Sea monitoring region IV. For COI, species level barcode coverage was 50.4% in GenBank and 42.4% for public sequences in BOLD. For 18S, species level coverage was 36.4% in GenBank and 27.1% in SILVA. To see whether rare species were underrepresented, we investigated the most commonly reported species as a separate dataset but found only minor coverage increases. We conclude that compared to global figures, barcode coverage is high for this area, but that a significant effort remains to fill barcode databases to levels that would make metabarcoding operational as a taxonomic tool, including for the most common macrofaunal taxa.

Bidragsytere

Jon Thomassen Hestetun

  • Tilknyttet:
    Forfatter
    ved NORCE Klima og miljø ved NORCE Norwegian Research Centre AS

Einar Bye-Ingebrigtsen

  • Tilknyttet:
    Forfatter
    ved NORCE Klima og miljø ved NORCE Norwegian Research Centre AS

R Henrik Nilsson

  • Tilknyttet:
    Forfatter
    ved Göteborgs universitet

Adrian G. Glover

  • Tilknyttet:
    Forfatter
    ved The Natural History Museum

Per-Otto Johansen

  • Tilknyttet:
    Forfatter
    ved NORCE Klima og miljø ved NORCE Norwegian Research Centre AS
1 - 5 av 6 | Neste | Siste »