Cristin-resultat-ID: 1823840
Sist endret: 5. februar 2021, 16:53
NVI-rapporteringsår: 2020
Resultat
Vitenskapelig artikkel
2020

Major-effect candidate genes identified in cultivated strawberry (Fragaria × ananassa Duch.) for ellagic acid deoxyhexoside and pelargonidin-3-O-malonylglucoside biosynthesis, key polyphenolic compounds

Bidragsytere:
  • Jahn Davik
  • Kjersti Aaby
  • Matteo Buti
  • Muath K Alsheikh
  • Nada Surbanovski
  • Stefan Martens
  • mfl.

Tidsskrift

Horticulture Research
ISSN 2662-6810
e-ISSN 2052-7276
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2020
Publisert online: 2020
Volum: 7
Artikkelnummer: 125
Open Access

Importkilder

Scopus-ID: 2-s2.0-85088862545

Beskrivelse Beskrivelse

Tittel

Major-effect candidate genes identified in cultivated strawberry (Fragaria × ananassa Duch.) for ellagic acid deoxyhexoside and pelargonidin-3-O-malonylglucoside biosynthesis, key polyphenolic compounds

Sammendrag

Strawberries are rich in polyphenols which impart health benefits when metabolized by the gut microbiome, including anti-inflammatory, neuroprotective, and antiproliferative effects. In addition, polyphenolic anthocyanins contribute to the attractive color of strawberry fruits. However, the genetic basis of polyphenol biosynthesis has not been extensively studied in strawberry. In this investigation, ripe fruits from three cultivated strawberry populations were characterized for polyphenol content using HPLC-DAD-MSn and genotyped using the iStraw35k array. GWAS and QTL analyses identified genetic loci controlling polyphenol biosynthesis. QTL were identified on four chromosomes for pelargonidin-3-O-malonylglucoside, pelargonidin-3-O-acetylglucoside, cinnamoyl glucose, and ellagic acid deoxyhexoside biosynthesis. Presence/absence of ellagic acid deoxyhexoside and pelargonidin-3-O-malonylglucoside was found to be under the control of major gene loci on LG1X2 and LG6b, respectively, on the F. × ananassa linkage maps. Interrogation of gene predictions in the F. vesca reference genome sequence identified a single candidate gene for ellagic acid deoxyhexoside biosynthesis, while seven malonyltransferase genes were identified as candidates for pelargonidin-3-O-malonylglucoside biosynthesis. Homologous malonyltransferase genes were identified in the F. × ananassa ‘Camarosa’ genome sequence but the candidate for ellagic acid deoxyhexoside biosynthesis was absent from the ‘Camarosa’ sequence. This study demonstrated that polyphenol biosynthesis in strawberry is, in some cases,under simple genetic control, supporting previous observations of the presence or absence of these compounds in strawberry fruits. It has also shed light on the mechanisms controlling polyphenol biosynthesis and enhanced the knowledge of these biosynthesis pathways in strawberry. The above findings will facilitate breeding for strawberries enriched in compounds with beneficial health effects.

Bidragsytere

Jahn Davik

  • Tilknyttet:
    Forfatter
    ved Divisjon for bioteknologi og plantehelse ved Norsk institutt for bioøkonomi

Kjersti Aaby

  • Tilknyttet:
    Forfatter
    ved Mat og helse ved NOFIMA

Matteo Buti

  • Tilknyttet:
    Forfatter
    ved Università degli Studi di Firenze

Muath K Alsheikh

  • Tilknyttet:
    Forfatter
    ved Institutt for plantevitenskap ved Norges miljø- og biovitenskapelige universitet
  • Tilknyttet:
    Forfatter
    ved Graminor AS

Nada Surbanovski

  • Tilknyttet:
    Forfatter
    ved National Institute of Agricultural Botany
1 - 5 av 8 | Neste | Siste »