Cristin-resultat-ID: 1847153
Sist endret: 21. januar 2021, 15:41
NVI-rapporteringsår: 2020
Resultat
Vitenskapelig artikkel
2020

Multigene expression assay for assessment of the immune status of Atlantic Salmon

Bidragsytere:
  • Aleksei Krasnov
  • Sergey Afanasyev
  • Stian Nylund og
  • Alexander Rebl

Tidsskrift

Genes
ISSN 2073-4425
e-ISSN 2073-4425
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2020
Publisert online: 2020
Volum: 11
Hefte: 11
Sider: 1 - 11
Artikkelnummer: 1236
Open Access

Importkilder

Scopus-ID: 2-s2.0-85093926265

Beskrivelse Beskrivelse

Tittel

Multigene expression assay for assessment of the immune status of Atlantic Salmon

Sammendrag

We report the development of a multigene gene expression assay on the BioMark HD platform for the evaluation of immune competence (ImCom) in farmed Atlantic salmon. The first version of the assay included 92 genes selected on the basis of transcriptome analyses in 54 trials that challenged the immune system; annotations were taken into account to represent the key pathways of innate and adaptive immunity. ImCom was tested on samples collected from seven independent projects. Fish were reared from the start feeding to eight months in the sea at eight units in different parts of Norway. Several tissues were analyzed. Linear discriminant analysis (LDA) showed that no more than 10 genes were required to separate groups, and a set of 46 immune genes was sufficient for any task. The second version of the assay was tested in the gills of two groups of high-performing healthy smolts and in groups with intermediate and high mortality rates (IM and HM, respectively). A set of 645 gill samples from clinically healthy Atlantic salmon was used as a reference. The IM group showed general suppression of immunity. All HM group salmon were above the threshold by the squared deviation from the reference. This group showed marked upregulation of genes involved in acute stress and inflammation: mmp-9, mmp-13, hsp70, il-1b, lect2, and cathelicidin. Further work will clarify the boundaries of the norm and explore various cases of impaired immunity.

Bidragsytere

Aleksei Krasnov

  • Tilknyttet:
    Forfatter
    ved Fiskehelse ved NOFIMA

Sergey Afanasyev

  • Tilknyttet:
    Forfatter
    ved Russian Academy of Sciences

Stian Nylund

  • Tilknyttet:
    Forfatter
    ved Pharmaq AS

Alexander Rebl

  • Tilknyttet:
    Forfatter
    ved Tyskland
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