Cristin-resultat-ID: 1888783
Sist endret: 25. mars 2021, 10:57
NVI-rapporteringsår: 2020
Resultat
Vitenskapelig artikkel
2020

Electrical Stimulation Induces Retinal Müller Cell Proliferation and Their Progenitor Cell Potential

Bidragsytere:
  • Sam Enayati
  • Karen Chang
  • Hamida Achour
  • Kin-Sang Cho
  • Fuyi Xu
  • Shuai Guo
  • mfl.

Tidsskrift

Cells
ISSN 2073-4409
e-ISSN 2073-4409
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2020
Volum: 9:781
Hefte: 3
Sider: 1 - 18
Open Access

Importkilder

Scopus-ID: 2-s2.0-85097839779

Beskrivelse Beskrivelse

Tittel

Electrical Stimulation Induces Retinal Müller Cell Proliferation and Their Progenitor Cell Potential

Sammendrag

Non-invasive electrical stimulation (ES) is increasingly applied to improve vision in untreatable eye conditions, such as retinitis pigmentosa and age-related macular degeneration. Our previous study suggested that ES promoted retinal function and the proliferation of progenitor-like glial cells in mice with inherited photoreceptor degeneration; however, the underlying mechanism remains obscure. Müller cells (MCs) are thought to be dormant residential progenitor cells that possess a high potential for retinal neuron repair and functional plasticity. Here, we showed that ES with a ramp waveform of 20 Hz and 300 µA of current was effective at inducing mouse MC proliferation and enhancing their expression of progenitor cell markers, such as Crx (cone–rod homeobox) and Wnt7, as well as their production of trophic factors, including ciliary neurotrophic factor. RNA sequencing revealed that calcium signaling pathway activation was a key event, with a false discovery rate of 5.33 × 10−8 (p = 1.78 × 10−10) in ES-mediated gene profiling changes. Moreover, the calcium channel blocker, nifedipine, abolished the observed effects of ES on MC proliferation and progenitor cell gene induction, supporting a central role of ES-induced Ca2+ signaling in the MC changes. Our results suggest that low-current ES may present a convenient tool for manipulating MC behavior toward neuroregeneration and repair.

Bidragsytere

Sam Enayati

  • Tilknyttet:
    Forfatter
    ved Drammen sykehus ved Vestre Viken HF
  • Tilknyttet:
    Forfatter
    ved Massachusetts Eye and Ear
  • Tilknyttet:
    Forfatter
    ved Avdeling for medisinsk biokjemi ved Oslo universitetssykehus HF
  • Tilknyttet:
    Forfatter
    ved Avdeling for medisinsk biokjemi ved Universitetet i Oslo

Karen Chang

  • Tilknyttet:
    Forfatter
    ved Massachusetts Eye and Ear

Hamida Achour

  • Tilknyttet:
    Forfatter
    ved Massachusetts Eye and Ear
  • Tilknyttet:
    Forfatter
    ved Klinikk for hode, hals og rekonstruktiv kirurgi ved Universitetet i Oslo

Kin-Sang Cho

  • Tilknyttet:
    Forfatter
    ved Massachusetts Eye and Ear

Fuyi Xu

  • Tilknyttet:
    Forfatter
    ved The University of Tennessee Health Science Center
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