Cristin-resultat-ID: 1889756
Sist endret: 8. mars 2021, 15:00
NVI-rapporteringsår: 2020
Resultat
Vitenskapelig artikkel
2020

The shift from early to late types of ribosomes in zebrafish development involves changes at a subset of rRNA 2′-O-Me sites

Bidragsytere:
  • Sowmya Ramachandran
  • Nicolai Krogh
  • Tor Erik Jørgensen
  • Steinar Daae Johansen
  • Henrik Nielsen og
  • Igor Babiak

Tidsskrift

RNA: A publication of the RNA Society
ISSN 1355-8382
e-ISSN 1469-9001
NVI-nivå 2

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2020
Volum: 26
Hefte: 12
Sider: 1919 - 1934

Importkilder

Scopus-ID: 2-s2.0-85096347553

Beskrivelse Beskrivelse

Tittel

The shift from early to late types of ribosomes in zebrafish development involves changes at a subset of rRNA 2′-O-Me sites

Sammendrag

During zebrafish development, an early type of rRNA is gradually replaced by a late type that is substantially different in sequence. We applied RiboMeth-seq to rRNA from developmental stages for profiling of 2′-O-Me, to learn if changes in methylation pattern were a component of the shift. We compiled a catalog of 2′-O-Me sites and cognate box C/D guide RNAs comprising 98 high-confidence sites, including 10 sites that were not known from other vertebrates, one of which was specific to late-type rRNA. We identified a subset of sites that changed in methylation status during development and found that some of these could be explained by availability of their cognate SNORDs. Sites that changed during development were enriched in the novel sites revealed in zebrafish. We propose that the early type of rRNA is a specialized form and that its structure and ribose methylation pattern may be an adaptation to features of development, including translation of specific maternal mRNAs.

Bidragsytere

Sowmya Ramachandran

  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet

Nicolai Krogh

  • Tilknyttet:
    Forfatter
    ved Københavns Universitet

Tor Erik Jørgensen

  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet

Steinar Daae Johansen

  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet

Henrik Nielsen

  • Tilknyttet:
    Forfatter
    ved Københavns Universitet
  • Tilknyttet:
    Forfatter
    ved Fakultet for biovitenskap og akvakultur ved Nord universitet
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