Cristin-resultat-ID: 190701
Sist endret: 21. oktober 2013, 12:13
Resultat
Vitenskapelig foredrag
2001

The nuclear receptor coactivators p/CIP and TIF2 that differentially regulate the PKA dependent transcriptional activity of Steroidogenic Factor-1

Bidragsytere:
  • Bente Børud
  • Gunnar Mellgren
  • Anne Lilybert J Jacob
  • Marit Bakke og
  • Johan Lund

Presentasjon

Navn på arrangementet: 37. Biokjemiske kontaktmøte. NBS Winter Meeting
Sted: Beitostølen
Dato fra: 1. januar 2001

Om resultatet

Vitenskapelig foredrag
Publiseringsår: 2001

Importkilder

Fdok-ID: 13217

Beskrivelse Beskrivelse

Tittel

The nuclear receptor coactivators p/CIP and TIF2 that differentially regulate the PKA dependent transcriptional activity of Steroidogenic Factor-1

Sammendrag

Steroidogenic factor-1 (SF-1) is a member of the nuclear receptor superfamily and plays an essential role in the development of endocrine organs. Coactivators and corepressors are important in the transcriptional regulation of gene expression by nuclear receptors. SRC-1 and TIF2 belongs to the SRC family of coactivators and are required for transcriptional regulation by several nuclear receptors including SF-1. Here we demonstrate that another SRC-related coactivator, p/CIP, interacts with SF-1, and that the interaction requires the carboxy-terminal activation function-2 (AF-2) domain of SF-1. By transfection studies performed in an adrenocortical cell line (Y1) and nonsteroidogenic COS-1 cells it is shown that both p/CIP and TIF2 potentates SF-1 mediated transactivation of a reporter construct under the control of the SF-1 binding site (CRS2) from the bovine CYP17 gene. Cotransfection with the catalytic subunit of cAMP-dependent protein kinase (PKA) demonstrated that increased PKA activity further stimulated the positive effect of p/CIP. In contrast, PKA markedly inhibited the positive effect of TIF2 on the transcriptional activity of SF-1. The AF-2 domain of SF-1 is required for the coactivating effect of both p/CIP and TIF2. However, the interaction between TIF2 and SF-1 differed from that of p/CIP and SF-1 with respect to the amino acid requirements within the AF-2 domain. Based on these results, we suggest that TIF2 acts by a mechanism distinct from that by which the other members of the SRC family to regulate the transcriptional activity of SF-1.

Bidragsytere

Aktiv cristin-person

Bente Børud

  • Tilknyttet:
    Forfatter
    ved Inst. for anatomi og cellebiologi ved Universitetet i Bergen

Gunnar Mellgren

  • Tilknyttet:
    Forfatter
    ved Inst. for anatomi og cellebiologi ved Universitetet i Bergen

Anne Lilybert J Jacob

  • Tilknyttet:
    Forfatter
    ved Inst. for anatomi og cellebiologi ved Universitetet i Bergen

Marit Bakke

  • Tilknyttet:
    Forfatter
Inaktiv cristin-person

Johan Lund

  • Tilknyttet:
    Forfatter
    ved Inst. for anatomi og cellebiologi ved Universitetet i Bergen
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