Cristin-resultat-ID: 1912897
Sist endret: 27. oktober 2021, 13:51
NVI-rapporteringsår: 2021
Resultat
Vitenskapelig artikkel
2021

Transcriptome responses in polar cod (Boreogadus saida) liver slice culture exposed to benzo[a]pyrene and ethynylestradiol: insights into anti-estrogenic effects

Bidragsytere:
  • Fekadu Yadetie
  • Nadja Rebecca Brun
  • Ireen Vieweg
  • Jasmine Nahrgang
  • Odd André Karlsen og
  • Anders Goksøyr

Tidsskrift

Toxicology in Vitro
ISSN 0887-2333
e-ISSN 1879-3177
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2021
Publisert online: 2021
Trykket: 2021
Volum: 75
Artikkelnummer: 105193
Open Access

Importkilder

Scopus-ID: 2-s2.0-85106354128

Beskrivelse Beskrivelse

Tittel

Transcriptome responses in polar cod (Boreogadus saida) liver slice culture exposed to benzo[a]pyrene and ethynylestradiol: insights into anti-estrogenic effects

Sammendrag

Polar cod (Boreogadus saida) is a key species in the arctic marine ecosystem vulnerable to effects of pollution, particularly from petroleum related activities. To facilitate studying the effects of those pollutants, we adapted a precision-cut liver slice culture protocol for this species. Using this system on board a research vessel, we studied gene expression in liver slice after exposure to the polycyclic aromatic hydrocarbon (PAH) benzo[a]pyrene (BaP), ethynylestradiol (EE2), and their mixtures, to map their molecular targets and examine possible anti-estrogenic effects of BaP. The exposure experiments were performed with BaP alone (0.1, 1, and 10 μM) or in combination with low concentrations of EE2 (5 nM) to mimic physiological estradiol levels in early vitellogenic female fish. Transcriptome analysis (RNA-seq) was performed after 72 h exposure in culture to map the genes and cellular pathways affected. The results provide a view of global transcriptome responses to BaP and EE2, which resulted in enrichment of many pathways such as the aryl hydrocarbon (Ahr) and estrogen receptor pathways. In the mixture exposure, BaP resulted in anti-estrogenic effects, shown by attenuation of EE2 activated transcription of many estrogen target genes. The results from this ex vivo experiment suggest that pollutants that activate the Ahr pathway such as the PAH compound BaP can result in anti-estrogenic effects that may lead to endocrine disruption in polar cod.

Bidragsytere

Fekadu Yadetie

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap (BIO) ved Universitetet i Bergen

Nadja Rebecca Brun

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap (BIO) ved Universitetet i Bergen
  • Tilknyttet:
    Forfatter
    ved Woods Hole Oceanographic Institution

Ireen Vieweg

  • Tilknyttet:
    Forfatter
    ved Institutt for arktisk og marin biologi ved UiT Norges arktiske universitet

Jasmine Magali Nahrgang-Berge

Bidragsyterens navn vises på dette resultatet som Jasmine Nahrgang
  • Tilknyttet:
    Forfatter
    ved Institutt for arktisk og marin biologi ved UiT Norges arktiske universitet

Odd André Karlsen

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap (BIO) ved Universitetet i Bergen
1 - 5 av 6 | Neste | Siste »