Cristin-resultat-ID: 1914765
Sist endret: 1. februar 2022, 16:05
NVI-rapporteringsår: 2021
Resultat
Vitenskapelig artikkel
2021

Long-Term Exposure to Nanosized TiO2 Triggers Stress Responses and Cell Death Pathways in Pulmonary Epithelial Cells

Bidragsytere:
  • Mayes Alswady-Hoff
  • Johanna Maria Samulin Erdem
  • Santosh Phuyal
  • Oskar Knittelfelder
  • Animesh Sharma
  • Davi de Miranda Fonseca
  • mfl.

Tidsskrift

International Journal of Molecular Sciences
ISSN 1661-6596
e-ISSN 1422-0067
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2021
Volum: 22
Hefte: 10
Artikkelnummer: 5349
Open Access

Importkilder

Scopus-ID: 2-s2.0-85106196595

Beskrivelse Beskrivelse

Tittel

Long-Term Exposure to Nanosized TiO2 Triggers Stress Responses and Cell Death Pathways in Pulmonary Epithelial Cells

Sammendrag

There is little in vitro data available on long-term effects of TiO2 exposure. Such data are important for improving the understanding of underlying mechanisms of adverse health effects of TiO2 . Here, we exposed pulmonary epithelial cells to two doses (0.96 and 1.92 µg/cm2 ) of TiO2 for 13 weeks and effects on cell cycle and cell death mechanisms, i.e., apoptosis and autophagy were determined after 4, 8 and 13 weeks of exposure. Changes in telomere length, cellular protein levels and lipid classes were also analyzed at 13 weeks of exposure. We observed that the TiO2 exposure increased the fraction of cells in G1-phase and reduced the fraction of cells in G2-phase, which was accompanied by an increase in the fraction of late apoptotic/necrotic cells. This corresponded with an induced expression of key apoptotic proteins i.e., BAD and BAX, and an accumulation of several lipid classes involved in cellular stress and apoptosis. These findings were further supported by quantitative proteome profiling data showing an increase in proteins involved in cell stress and genomic maintenance pathways following TiO2 exposure. Altogether, we suggest that cell stress response and cell death pathways may be important molecular events in long-term health effects of TiO2

Bidragsytere

Mayes Alswady-Hoff

  • Tilknyttet:
    Forfatter
    ved Statens arbeidsmiljøinstitutt

Johanna Samulin-Erdem

Bidragsyterens navn vises på dette resultatet som Johanna Maria Samulin Erdem
  • Tilknyttet:
    Forfatter
    ved Statens arbeidsmiljøinstitutt

Santosh Phuyal

  • Tilknyttet:
    Forfatter
    ved Statens arbeidsmiljøinstitutt
  • Tilknyttet:
    Forfatter
    ved Avdeling for molekylærmedisin ved Universitetet i Oslo

Oskar Knittelfelder

  • Tilknyttet:
    Forfatter
    ved Max-Planck-Institut für molekulare Genetik

Animesh Sharma

  • Tilknyttet:
    Forfatter
    ved Helse Midt-Norge
  • Tilknyttet:
    Forfatter
    ved Institutt for klinisk og molekylær medisin ved Norges teknisk-naturvitenskapelige universitet
1 - 5 av 9 | Neste | Siste »