Cristin-resultat-ID: 1918905
Sist endret: 14. oktober 2021, 11:09
NVI-rapporteringsår: 2021
Resultat
Vitenskapelig artikkel
2021

Accumulation dynamics of transcripts and proteins of cold-responsive genes in Fragaria vesca genotypes of differing cold tolerance

Bidragsytere:
  • Isam Fattash
  • Zachary Deitch
  • Relindis Ghai Njah
  • Nelson Uchechukwu Osuagwu
  • Vera Mageney
  • Robert Charles Wilson
  • mfl.

Tidsskrift

International Journal of Molecular Sciences
ISSN 1422-0067
e-ISSN 1422-0067
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2021
Volum: 22
Hefte: 11
Sider: 1 - 21
Artikkelnummer: 6124
Open Access

Importkilder

Scopus-ID: 2-s2.0-85107276589

Beskrivelse Beskrivelse

Tittel

Accumulation dynamics of transcripts and proteins of cold-responsive genes in Fragaria vesca genotypes of differing cold tolerance

Sammendrag

Identifying and characterizing cold responsive genes in Fragaria vesca associated with or responsible for low temperature tolerance is a vital part of strawberry cultivar development. In this study we have investigated the transcript levels of eight genes, two dehydrin genes, three putative ABA-regulated genes, two cold–inducible CBF genes and the alcohol dehydrogenase gene, extracted from leaf and crown tissues of three F. vesca genotypes that vary in cold tolerance. Transcript levels of the CBF/DREB1 transcription factor FvCBF1E exhibited stronger cold up-regulation in comparison to FvCBF1B.1 in all genotypes. Transcripts of FvADH were highly up-regulated in both crown and leaf tissues from all three genotypes. In the ‘ALTA’ genotype, FvADH transcripts were significantly higher in leaf than crown tissues and more than 10 to 20-fold greater than in the less cold-tolerant ‘NCGR1363’ and ‘FDP817’ genotypes. FvGEM, containing the conserved ABRE promoter element, transcript was found to be cold-regulated in crowns. Direct comparison of the kinetics of transcript and protein accumulation of dehydrins was scrutinized. In all genotypes and organs, the changes of XERO2 transcript levels generally preceded protein changes, while levels of COR47 protein accumulation preceded the increases in COR47 RNA in ‘ALTA’ crowns.

Bidragsytere

Isam Fattash

  • Tilknyttet:
    Forfatter
    ved Jordan

Zachary Deitch

  • Tilknyttet:
    Forfatter
    ved Indiana University-Purdue University at Indianapolis

Relindis Ghai Njah

  • Tilknyttet:
    Forfatter
    ved Institutt for bioteknologi ved Høgskolen i Innlandet

Nelson Uchechukwu Osuagwu

  • Tilknyttet:
    Forfatter
    ved Klinisk institutt 1 ved Universitetet i Bergen

Vera Mageney

  • Tilknyttet:
    Forfatter
    ved Carl von Ossietzky Universität Oldenburg
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