Cristin-resultat-ID: 1923573
Sist endret: 28. januar 2022, 12:26
NVI-rapporteringsår: 2021
Resultat
Vitenskapelig artikkel
2021

Biosynthesis of Long-Chain Polyunsaturated Fatty Acids in Marine Gammarids: Molecular Cloning and Functional Characterisation of Three Fatty Acyl Elongases

Bidragsytere:
  • Alberto Ribes-Navarro
  • Juan Carlos Navarro
  • Francisco Hontoria
  • Naoki Kabeya
  • Inger Beate Standal
  • Jan Ove Evjemo
  • mfl.

Tidsskrift

Marine Drugs
ISSN 1660-3397
e-ISSN 1660-3397
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2021
Volum: 19
Hefte: 4
Artikkelnummer: 226
Open Access

Importkilder

Scopus-ID: 2-s2.0-85105127387

Beskrivelse Beskrivelse

Tittel

Biosynthesis of Long-Chain Polyunsaturated Fatty Acids in Marine Gammarids: Molecular Cloning and Functional Characterisation of Three Fatty Acyl Elongases

Sammendrag

Long-chain (C20–24) polyunsaturated fatty acids (LC-PUFAs) are essential nutrients that are mostly produced in marine ecosystems. Previous studies suggested that gammarids have some capacity to endogenously produce LC-PUFAs. This study aimed to investigate the repertoire and functions of elongation of very long-chain fatty acid (Elovl) proteins in gammarids. Our results show that gammarids have, at least, three distinct elovl genes with putative roles in LC-PUFA biosynthesis. Phylogenetics allowed us to classify two elongases as Elovl4 and Elovl6, as they were bona fide orthologues of vertebrate Elovl4 and Elovl6. Moreover, a third elongase was named as “Elovl1/7-like” since it grouped closely to the Elovl1 and Elovl7 found in vertebrates. Molecular analysis of the deduced protein sequences indicated that the gammarid Elovl4 and Elovl1/7-like were indeed polyunsaturated fatty acid (PUFA) elongases, whereas Elovl6 had molecular features typically found in non-PUFA elongases. This was partly confirmed in the functional assays performed on the marine gammarid Echinogammarus marinus Elovl, which showed that both Elovl4 and Elovl1/7-like elongated PUFA substrates ranging from C18 to C22. E. marinus Elovl6 was only able to elongate C18 PUFA substrates, suggesting that this enzyme does not play major roles in the LC-PUFA biosynthesis of gammarids.

Bidragsytere

Alberto Ribes-Navarro

  • Tilknyttet:
    Forfatter
    ved Instituto de Acuicultura Torre de la Sal

Juan Carlos Navarro

  • Tilknyttet:
    Forfatter
    ved Instituto de Acuicultura Torre de la Sal

Francisco Hontoria

  • Tilknyttet:
    Forfatter
    ved Instituto de Acuicultura Torre de la Sal

Naoki Kabeya

  • Tilknyttet:
    Forfatter
    ved Tokyo Kaiyo Daigaku

Inger Beate Standal

  • Tilknyttet:
    Forfatter
    ved Fiskeri og ny biomarin industri ved SINTEF Ocean
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