Cristin-resultat-ID: 1927899
Sist endret: 14. desember 2021, 11:48
NVI-rapporteringsår: 2021
Resultat
Vitenskapelig artikkel
2021

Streptomyces poriferorum sp. nov., a novel marine sponge-derived Actinobacteria species expressing anti-MRSA activity

Bidragsytere:
  • Megan Sandoval-Powers
  • Stanislava Králová
  • Giang-Son Nguyen
  • Dorelle V. Fawwal
  • Kristin Fløgstad Degnes
  • Anna Lewin
  • mfl.

Tidsskrift

Systematic and Applied Microbiology
ISSN 0723-2020
e-ISSN 1618-0984
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2021
Publisert online: 2021
Trykket: 2021
Volum: 44
Hefte: 5
Artikkelnummer: 126244

Importkilder

Scopus-ID: 2-s2.0-85112347147

Beskrivelse Beskrivelse

Tittel

Streptomyces poriferorum sp. nov., a novel marine sponge-derived Actinobacteria species expressing anti-MRSA activity

Sammendrag

Marine sponges represent a rich source of uncharacterized microbial diversity, and many are host to microorganisms that produce biologically active specialized metabolites. Here, a polyphasic approach was used to characterize two Actinobacteria strains, P01-B04T and P01-F02, that were isolated from the marine sponges Geodia barretti (Bowerbank, 1858) and Antho dichotoma (Esper, 1794), respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strains P01-B04T and P01-F02 are closely related to Streptomyces beijiangensis DSM 41794T, Streptomyces laculatispora NRRL B-24909T, and Streptomyces brevispora NRRL B-24910T. The two strains showed nearly identical 16S rRNA gene sequences (99.93%), and the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) relatedness values were 99.96% and 99.6%, respectively, suggesting that these strains are affiliated with the same species. Chemotaxonomic and culture characteristics of both strains were also consistent with the genus Streptomyces, while phenotypic properties, genome-based comparisons, and phylogenomic analyses distinguished strains P01-B04T and P01-F02 from their closest phylogenetic relatives. In silico analysis predicted that the 8.9 Mb genome of P01-B04T contains at least 41 biosynthetic gene clusters (BGCs) encoding secondary metabolites, indicating that this strain could express diverse bioactive metabolites; in support of this prediction, this strain expressed antibacterial activity against Gram-positive bacteria including a clinical isolate of methicillin-resistant Staphylococcus aureus (MRSA) EAMC30. Based on these results, the marine sponge-associated isolates represent a novel species of the genus Streptomyces, for which the name Streptomyces poriferorum sp. nov. is proposed, with P01-B04T (=DSM 111306T = CCM 9048T) as the type strain.

Bidragsytere

Megan Sandoval-Powers

  • Tilknyttet:
    Forfatter
    ved Auburn University

Stanislava Králová

  • Tilknyttet:
    Forfatter
    ved Masarykova univerzita
  • Tilknyttet:
    Forfatter
    ved Auburn University

Giang-Son Nguyen

  • Tilknyttet:
    Forfatter
    ved Bioteknologi og nanomedisin ved SINTEF AS

Dorelle V. Fawwal

  • Tilknyttet:
    Forfatter
    ved Auburn University

Kristin Fløgstad Degnes

  • Tilknyttet:
    Forfatter
    ved Bioteknologi og nanomedisin ved SINTEF AS
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