Cristin-resultat-ID: 1928187
Sist endret: 14. januar 2022, 10:18
NVI-rapporteringsår: 2021
Resultat
Vitenskapelig artikkel
2021

Reproducible lipid alterations in patient-derived breast cancer xenograft FFPE tissue identified with MALDI MSI for pre-clinical and clinical application

Bidragsytere:
  • Vanna Denti
  • Maria Karoline Andersen
  • Andrew Smith
  • Anna M. Bofin
  • Anna Nordborg
  • Fulvio Magni
  • mfl.

Tidsskrift

Metabolites
ISSN 2218-1989
e-ISSN 2218-1989
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2021
Publisert online: 2021
Volum: 11
Hefte: 9
Artikkelnummer: 577
Open Access

Importkilder

Scopus-ID: 2-s2.0-85114255731

Beskrivelse Beskrivelse

Tittel

Reproducible lipid alterations in patient-derived breast cancer xenograft FFPE tissue identified with MALDI MSI for pre-clinical and clinical application

Sammendrag

The association between lipid metabolism and long-term outcomes is relevant for tumor diagnosis and therapy. Archival material such as formalin-fixed and paraffin embedded (FFPE) tissues is a highly valuable resource for this aim as it is linked to long-term clinical follow-up. Therefore, there is a need to develop robust methodologies able to detect lipids in FFPE material and correlate them with clinical outcomes. In this work, lipidic alterations were investigated in patient-derived xenograft of breast cancer by using a matrix-assisted laser desorption ionization mass spectrometry (MALDI MSI) based workflow that included antigen retrieval as a sample preparation step. We evaluated technical reproducibility, spatial metabolic differentiation within tissue compartments, and treatment response induced by a glutaminase inhibitor (CB-839). This protocol shows a good inter-day robustness (CV = 26 ± 12%). Several lipids could reliably distinguish necrotic and tumor regions across the technical replicates. Moreover, this protocol identified distinct alterations in the tissue lipidome of xenograft treated with glutaminase inhibitors. In conclusion, lipidic alterations in FFPE tissue of breast cancer xenograft observed in this study are a step-forward to a robust and reproducible MALDI-MSI based workflow for pre-clinical and clinical applications.

Bidragsytere

Vanna Denti

  • Tilknyttet:
    Forfatter
    ved Università degli Studi di Milano-Bicocca

Maria Karoline Andersen

  • Tilknyttet:
    Forfatter
    ved Institutt for sirkulasjon og bildediagnostikk ved Norges teknisk-naturvitenskapelige universitet

Andrew Smith

  • Tilknyttet:
    Forfatter
    ved Università degli Studi di Milano-Bicocca

Anna Mary Bofin

Bidragsyterens navn vises på dette resultatet som Anna M. Bofin
  • Tilknyttet:
    Forfatter
    ved Institutt for klinisk og molekylær medisin ved Norges teknisk-naturvitenskapelige universitet

Anna Nordborg

  • Tilknyttet:
    Forfatter
    ved Bioteknologi og nanomedisin ved SINTEF AS
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