Cristin-resultat-ID: 1945063
Sist endret: 18. februar 2022, 10:48
NVI-rapporteringsår: 2021
Resultat
Vitenskapelig artikkel
2021

Removal of large viruses and their dispersal through fecal pellets of the appendicularian Oikopleura dioica during Emiliania huxleyi bloom conditions

Bidragsytere:
  • Kyle Mayers
  • Janice Lawrence
  • Katrine Sandnes Skaar
  • Joachim Paul Töpper
  • Elzbieta Anna Petelenz-Kurdziel
  • Marius Rydningen Saltvedt
  • mfl.

Tidsskrift

Limnology and Oceanography
ISSN 0024-3590
e-ISSN 1939-5590
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2021
Publisert online: 2021
Volum: 66
Hefte: 11
Sider: 3963 - 3975

Importkilder

Scopus-ID: 2-s2.0-85116365279

Beskrivelse Beskrivelse

Tittel

Removal of large viruses and their dispersal through fecal pellets of the appendicularian Oikopleura dioica during Emiliania huxleyi bloom conditions

Sammendrag

Despite their importance in shaping the structure and function of marine microbial food webs, little is known about factors regulating marine virus abundance. Previous work demonstrated clearance of laboratory-cultured Emiliania huxleyi virus by the appendicularian Oikopleura dioica; however, the applicability of this interaction to natural virus assemblages was not investigated. Here, we conducted controlled laboratory experiments using O. dioica and mesocosm water containing natural virus assemblages with high densities of virus, and measured removal of virus by O. dioica using both flow cytometry and molecular methods. Bayesian models based on flow cytometry quantification of virus particles demonstrated efficient removal of viruses (mean 90.3 mL ind−1 d−1), with a clearance efficiency of 42.6% relative to food algae. Molecular detection of virus removal by quantification of viral mcp gene copies revealed a mean clearance rate of 68.1 mL ind−1 d−1. Fecal pellets from these experiments demonstrated that viruses in fecal pellets retain infectivity despite passage through the O. dioica gut. Shotgun metavirome analysis demonstrated O. dioica removal of large virus groups, notably the Phycodnaviridae. The results demonstrate the removal of E. huxleyi virus from natural virus assemblages by O. dioica and the maintenance of viral infectivity when incorporated into fecal pellets, prompting further investigation on the fate of fecal-packaged viruses and their impact on host dynamics. Furthermore, our results indicate the generality of this interaction for other large algal viruses, raising questions about the implications of this mechanism of marine virus redistribution on the broader marine virus community.

Bidragsytere

Kyle Mayers

  • Tilknyttet:
    Forfatter
    ved NORCE Klima og miljø ved NORCE Norwegian Research Centre AS

Janice Lawrence

  • Tilknyttet:
    Forfatter
    ved University of New Brunswick

Katrine Sandnes Skaar

  • Tilknyttet:
    Forfatter
    ved NORCE Klima og miljø ved NORCE Norwegian Research Centre AS

Joachim Paul Töpper

  • Tilknyttet:
    Forfatter
    ved NINA Bergen ved Norsk institutt for naturforskning

Elzbieta Anna Petelenz-Kurdziel

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap ved Universitetet i Bergen
1 - 5 av 10 | Neste | Siste »