Cristin-resultat-ID: 2008275
Sist endret: 10. mars 2022, 13:40
Resultat
Vitenskapelig artikkel
2021

Small-Scale Comparative Genomic Analysis of Listeria monocytogenes Isolated from Environments of Salmon Processing Plants and Human Cases in Norway

Bidragsytere:
  • Trond Karsten Løvdal
  • Lin Cathrine T. Brandal
  • Arvind Sundaram
  • Umaer Naseer
  • Bjørn Roth og
  • Bjørn Tore Lunestad

Tidsskrift

Hygiene
ISSN 2673-947X
e-ISSN 2673-947X
NVI-nivå 1

Om resultatet

Vitenskapelig artikkel
Publiseringsår: 2021
Publisert online: 2021
Volum: 1
Sider: 43 - 55
Open Access

Beskrivelse Beskrivelse

Tittel

Small-Scale Comparative Genomic Analysis of Listeria monocytogenes Isolated from Environments of Salmon Processing Plants and Human Cases in Norway

Sammendrag

Listeria monocytogenes is a food-borne bacterium that give rise to the potentially life-threatening disease listeriosis. Listeriosis has been mandatorily notifiable in Norway since 1991. All clinical L. monocytogenes isolates are sent to the Norwegian Institute of Public Health (NIPH) for typing. Since 2005 Multi-Locus Variable number tandem repeats Analysis (MLVA) has been used for typing but was recently replaced by whole genome sequencing using core genome Multi-Locus Sequence Typing (cgMLST). In the present study, L. monocytogenes isolates collected at salmon processing plants in Norway in 2007 (n = 12) and 2015 (n = 14) were first subject to MLVA. Twelve clinical L. monocytogenes isolates with matching MLVA profile and sampling time were selected from the strain collection at NIPH. Twenty-one isolates from the salmon processing plants and all clinical isolates (n = 12) were whole genome sequenced and compared using cgMLST and in silico detection of virulence genes. cgMLST revealed four pairs of environmental–human isolates with ≤10 allelic differences over 1708 genes, indicating that they may be assigned as clonal, with the implication that they are descended from the same recent ancestor. No relevant difference in carriage of virulence genes was found between environmental or human isolates. The present study shows that L. monocytogenes strains that genetically resemble contemporary isolates from human listeriosis circulate in Norwegian salmon slaughterhouses, and carry the same virulence genes.

Bidragsytere

Trond Karsten Løvdal

  • Tilknyttet:
    Forfatter
    ved Prosessteknologi ved NOFIMA

Lin Cathrine T. Brandal

  • Tilknyttet:
    Forfatter
    ved Avdeling for smittevern og beredskap ved Folkehelseinstituttet

Arvind Sundaram

  • Tilknyttet:
    Forfatter
    ved Avdeling for medisinsk genetikk ved Oslo universitetssykehus HF

Umaer Naseer

  • Tilknyttet:
    Forfatter
    ved Folkehelseinstituttet

Bjørn Roth

  • Tilknyttet:
    Forfatter
    ved Prosessteknologi ved NOFIMA
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