Cristin-resultat-ID: 2027753
Sist endret: 27. mai 2022, 14:40
Resultat
Poster
2022

Transcriptome responses in copepods Calanus finmarchicus, Calanus glacialis and Calanus hyperboreus exposed to phenanthrene and benzo[a]pyrene

Bidragsytere:
  • Fekadu Yadetie
  • Nadja Rebecca Brun
  • Julia Giebichenstein
  • Ketil Hylland
  • Katrine Borgå
  • Odd André Karlsen
  • mfl.

Presentasjon

Navn på arrangementet: Pollutant Responses In Marine Organisms (PRIMO21), 22-25 May 2022, Gothenburg, Sweden
Sted: Gothenburg
Dato fra: 22. mai 2022
Dato til: 25. mai 2022

Arrangør:

Arrangørnavn: University of Gothenburg

Om resultatet

Poster
Publiseringsår: 2022

Beskrivelse Beskrivelse

Tittel

Transcriptome responses in copepods Calanus finmarchicus, Calanus glacialis and Calanus hyperboreus exposed to phenanthrene and benzo[a]pyrene

Sammendrag

With the warming Arctic allowing more human activities, the marine arctic ecosystems are increasingly at risk of exposure to pollutants from offshore petroleum-related industries. Thus, it is important to understand how arctic and sub-arctic pelagic organisms respond to crude oil-related contaminants such as polycyclic aromatic hydrocarbons (PAHs). The copepod species Calanus finmarchicus, Calanus glacialis and Calanus hyperboreus are the most abundant pelagic zooplankton in the northern Barents Sea and represent key links in the arctic marine food web. The three species were sampled during a research cruise on the Norwegian vessel RV Kronprins Haakon in August 2019 and subsequently exposed onboard at 3.5 ˚C (C. finmarchicus and C. glacialis), and 1.5 ˚C (C. hyperboreus) for 72 h to phenanthrene (Phe; 0.1 µM) and benzo[a]pyrene (BaP; 0.1 µM), representing low and high molecular weight PAHs, respectively. Global transcriptome (RNA-seq) analysis revealed differential expression of several genes involved in many cellular pathways. In C. finmarchicus and C. glacialis, the PAH exposure resulted in up-regulation of genes encoding enzymes in xenobiotic biotransformation processes, particularly the phase II cytosolic sulfonation system that includes 3'-phosphoadenosine 5'-phosphosulfate synthase (PAPSS) and sulfotransferases (SULTs). The sulfonation pathway genes were more strongly induced by BaP than Phe in C. finmarchicus and C. glacialis, but were not affected in C. hyperboreus. However, a larger number of genes and pathways were modulated by the PAHs in C. hyperboreus including genes encoding xenobiotic biotransformation and lipid metabolism enzymes, suggesting stronger responses to crude oil exposure in this high-Arctic species. The results suggest that cytosolic sulfonation is a major phase II conjugation pathway for PAHs in C. finmarchicus and C. glacialis. Further, the overall results from the transcriptome analysis provide insights into mechanisms involved in PAH response and metabolism in the three Calanus species most dominant in the region. Funding: The study was funded by the Research Council of Norway, through the Nansen Legacy Project (276730).

Bidragsytere

Fekadu Yadetie

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap (BIO) ved Universitetet i Bergen

Nadja Rebecca Brun

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap (BIO) ved Universitetet i Bergen

Julia Giebichenstein

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap ved Universitetet i Oslo
Aktiv cristin-person

Ketil Hylland

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap ved Universitetet i Oslo

Katrine Borgå

  • Tilknyttet:
    Forfatter
    ved Institutt for biovitenskap ved Universitetet i Oslo
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