Cristin-resultat-ID: 2040146
Sist endret: 7. oktober 2022, 10:56
Resultat
Rapport
2022

Safety evaluation of the food enzyme glucose oxidase from the genetically modified Trichoderma reesei strain AR-352

Bidragsytere:
  • Claude Lambré
  • José Manuel Barat Baviera
  • Claudia Bolognesi
  • Pier Sandro Cocconcelli
  • Riccardo Crebelli
  • David Michael Gott
  • mfl.

Utgiver/serie

Utgiver

European Food Safety Authority

Serie

EFSA Journal
ISSN 1831-4732
NVI-nivå 1

Om resultatet

Rapport
Publiseringsår: 2022
Volum: 20
Hefte: 7/7372
Antall sider: 15
Open Access

Klassifisering

Fagfelt (NPI)

Fagfelt: Biovitenskap
- Fagområde: Realfag og teknologi

Beskrivelse Beskrivelse

Tittel

Safety evaluation of the food enzyme glucose oxidase from the genetically modified Trichoderma reesei strain AR-352

Sammendrag

The food enzyme glucose oxidase (β-d-glucose:oxygen 1-oxidoreductase; EC 1.1.3.4) is produced with the genetically modified Trichoderma reesei strain AR-352 by AB Enzymes GmbH. The genetic modifications do not give rise to safety concerns. The food enzyme is free from viable cells of the production organism, but the absence of its recombinant DNA could not be established. The food enzyme is intended to be used in four food manufacturing processes, namely baking processes, cereal-based processes, grain treatment for the production of starch and gluten fractions, and egg processing. Since residual amounts of total organic solids (TOS) are removed by repeated washing during the production of starch and gluten, dietary exposure was calculated only for the remaining three processes. Dietary exposure to the food enzyme–TOS was estimated to be up to 0.13 mg TOS/kg body weight (bw) per day in European populations. Genotoxicity tests did not indicate a safety concern. The systemic toxicity was assessed by means of a repeated dose 90-day oral toxicity study in rats. The Panel identified a no observed adverse effect level of 1,000 mg TOS/kg bw per day, the highest dose tested, which when compared with the estimated dietary exposure, results in a margin of exposure of more than 7,800. A search for similarity of the amino acid sequence of the food enzyme to known allergens was made and one match was found. The Panel considered that, under the intended conditions of use, the risk of allergic sensitisation and elicitation reactions by dietary exposure cannot be excluded, but the likelihood for this to occur is considered to be low. Based on the data provided, the Panel concluded that this food enzyme does not give rise to safety concerns, under the intended conditions of use.

Bidragsytere

Claude Lambré

  • Tilknyttet:
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José Manuel Barat Baviera

  • Tilknyttet:
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Claudia Bolognesi

  • Tilknyttet:
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Pier Sandro Cocconcelli

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Riccardo Crebelli

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