Cristin-resultat-ID: 2042471
Sist endret: 11. august 2022, 14:21
Resultat
Doktorgradsavhandling
2022

Liver organoids, mass spectrometry and separation science

Bidragsytere:
  • Frøydis Sved Skottvoll

Utgiver/serie

Utgiver

Department of Chemistry, Faculty of Mathematics and Natural Sciences, University of Oslo

Serie

Series of dissertations submitted to the Faculty of Mathematics and Natural Sciences, University of Oslo.
ISSN 1501-7710
NVI-nivå 0

Om resultatet

Doktorgradsavhandling
Publiseringsår: 2022
Volum: 2022
Hefte: 2493

Klassifisering

Fagfelt (NPI)

Fagfelt: Kjemi
- Fagområde: Realfag og teknologi

Beskrivelse Beskrivelse

Tittel

Liver organoids, mass spectrometry and separation science

Sammendrag

Organoids are predicted to change the future of medicine by acting as an in vitro 3D representation of human organ functionality. Organoids are grown from patient derived stem cells or adult tissue, which promise to enable new and faster drug discovery, disease modeling and personalized medicine under more realistic conditions. Organoids are still at the developmental stage and fundamental questions concerning the functionality of the developed organoids remain to be answered. Compared to other biosampling techniques, mass spectrometry (MS) has rarely been used in organoid analyses. This thesis leverages MS and separation science to analyze the biological properties of liver organoids, spanning from protein identification to small molecule drug metabolite detection. With the aim of developing selective, high throughput analyses and online biosampling integration, both conventional and cutting edge electromembrane extraction (EME) sample preparation approaches were explored. Using bioanalytical tools including MS and separation science, we could establish that the liver organoids displayed liver organ functionality. The bioanalytical strategies we developed for liver organoid analyses contribute to greater insight into organoid response and functionality and could be important tools in future organoid development. Furthermore, online organoid integration using electromembrane extraction developed here could be used as a starting point towards developing future organ-on-a-chip systems integrated with mass spectrometry, which would be valuable in drug development, disease modeling, and personalized medicine.

Bidragsytere

Frøydis Sved Skottvoll

  • Tilknyttet:
    Forfatter
    ved Kjemisk institutt ved Universitetet i Oslo
  • Tilknyttet:
    Forfatter
    ved Smart Sensors and Microsystems ved SINTEF AS

Steven Ray Haakon Wilson

  • Tilknyttet:
    Veileder
    ved Kjemisk livsvitenskap ved Universitetet i Oslo

Ola Nilsen

  • Tilknyttet:
    Veileder
    ved Uorganisk materialkjemi ved Universitetet i Oslo
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